MYD88 Protein (AA 148-296) (His tag)
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- 抗原 See all MYD88 蛋白
- MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))
- 蛋白类型
- Recombinant
- 产品特性
- AA 148-296
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This MYD88 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys), Functional Studies (Func)
- 序列
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TL DDPLGQTPEL FDAFICYCPN DIEFVQEMIR QLEQTDYRLK LCVSDRDVLP GTCVWSIASE LIEKRCRRMV VVVSDDYLQS KECDFQTKFA LSLSPGVQQK RLIPIKYKAM KKDFPSILRF ITICDYTNPC TKSWFWTRLA KALSLP
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse MYD88 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product MYD88 蛋白
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Myeloid Differentiation Primary Response Gene (88) (MYD88) (AA 31-130) protein (GST tag)
MYD88 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Myeloid Differentiation Primary Response Gene (88) (MYD88) (AA 1-296) protein (GST tag)MYD88 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Myeloid Differentiation Primary Response Gene (88) (MYD88) (AA 1-296) protein (Strep Tag)Crystallography grade MYD88 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Myeloid Differentiation Primary Response Gene (88) (MYD88) (AA 176-280) protein (His tag,Strep II tag)MYD88 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant ~95 % WB, ELISA, MS
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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The protein will be expressed with a N-terminal GST-His-Tag. The protein is expressed with GST-tag, undergoes thrombin cleavage. The final product is the human Myd88 TIR domain with a N-terminal -His-Tag
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))
- 别名
- MYD88 (MYD88 产品)
- 别名
- MYD88D Protein, XMyD88 Protein, myd88d Protein, MGC84928 Protein, CG2078 Protein, DMMYD88 Protein, DmMyD88 Protein, DmMyd88 Protein, Dmel\\CG2078 Protein, EP(2)2535 Protein, Kra Protein, LD20892 Protein, MYD88 Protein, MyD88 Protein, Myd88F Protein, dMyD88 Protein, dMyd88 Protein, kra Protein, myd88 Protein, zgc:103541 Protein, GB12344 Protein, NV10640 Protein, myd88-a Protein, myd88-b Protein, myeloid differentiation primary response 88 Protein, myeloid differentiation primary response 88 S homeolog Protein, myeloid differentiation primary response gene 88 Protein, CG2078 gene product from transcript CG2078-RB Protein, myeloid differentiation primary response protein MyD88-A Protein, myeloid differentiation primary response protein MyD88 Protein, myeloid differentiation factor 88 Protein, myeloid differentiation primary response 88 L homeolog Protein, MYD88 Protein, myd88.S Protein, Myd88 Protein, myd88 Protein, LOC413194 Protein, LOC100118553 Protein, LOC575153 Protein, myd88.L Protein
- 背景
- Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Isoform 2 is defective in its ability to induce IRAK phosphorylation and NF-kappa-B activation and can function as a negative regulator of activation by IL-1 or lipopolysaccharide (LPS). Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine. {ECO:0000269|PubMed:11909531, ECO:0000269|PubMed:17018642, ECO:0000269|PubMed:17635956, ECO:0000269|PubMed:18941239, ECO:0000269|PubMed:21998396, ECO:0000269|PubMed:2374694, ECO:0000269|PubMed:9575168, ECO:0000269|PubMed:9697844}
- UniProt
- P22366
- 途径
- NF-kappaB Signaling, TLR signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades
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