MKNK2 Protein (AA 1-459) (Strep Tag)
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- 抗原 See all MKNK2 蛋白
- MKNK2 (MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-459
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This MKNK2 protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MVQKRTAELQ GFHRSFKGQN PFELAFSLDL AQHRDSDFSP QCEARPDMPS SQPIDIPDAK KRGRKKKRCR ATDSFSGRFE DVYQLQEDVL GEGAHARVQT CVNLITNQEY AVKIIEKQLG HIRSRVFREV EMLYQCQGHR NVLELIEFFE EEDRFYLVFE KMRGGSILSH IHRRRHFNEL EASVVVQDVA SALDFLHNKG IAHRDLKPEN ILCEHPNQVS PVKICDFDLG SGIKLNGDCS PISTPELLTP CGSAEYMAPE VVEAFSEEAS IYDKRCDLWS LGVILYILLS GYPPFVGHCG SDCGWDRGEA CPACQNMLFE SIQEGKYEFP DKDWSHISFA AKDLISKLLV RDAKQRLSAA QVLQHPWVQG CAPENTLPTP LVLQRNSCAK DLTSFAAEAI AMNRQLAQCE EDAGQDQPVV IRATSRCLQL SPPSQSKLAQ RRQRASLSAT PVVLVGDRA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product MKNK2 蛋白
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MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (Thr379Asp-Mutant) (Active) protein (GST tag)
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MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (AA 1-465) protein (Strep Tag)Crystallography grade MKNK2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (AA 1-158) protein (GST tag)MKNK2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (AA 41-158) protein (GST tag)MKNK2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) protein (His tag)MKNK2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)MKNK2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- MKNK2 (MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2))
- 别名
- Mknk2 (MKNK2 产品)
- 别名
- GPRK7 Protein, MNK2 Protein, gprk7 Protein, mnk2 Protein, 2010016G11Rik Protein, Gprk7 Protein, Mnk2 Protein, mknk2 Protein, wu:fb37e05 Protein, wz5090 Protein, fi34c11 Protein, wu:fi34c11 Protein, wu:fi41e12 Protein, zgc:55587 Protein, MAP kinase interacting serine/threonine kinase 2 Protein, MAP kinase-interacting serine/threonine kinase 2 Protein, MAP kinase interacting serine/threonine kinase 2b Protein, MAP kinase interacting serine/threonine kinase 2 L homeolog Protein, MAP kinase interacting serine/threonine kinase 2a Protein, MKNK2 Protein, mknk2 Protein, Mknk2 Protein, mknk2b Protein, mknk2.L Protein, mknk2a Protein
- 背景
- MAP kinase-interacting serine/threonine-protein kinase 2 (EC 2.7.11.1) (MAP kinase signal-integrating kinase 2) (MAPK signal-integrating kinase 2) (Mnk2),FUNCTION: Serine/threonine-protein kinase that phosphorylates SFPQ/PSF, HNRNPA1 and EIF4E. May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Required for mediating PP2A-inhibition-induced EIF4E phosphorylation. Triggers EIF4E shuttling from cytoplasm to nucleus. Enhances the formation of EIF4F complex in pachytene spermatocytes, thus promoting mRNA translation during spermatogenesis. Displays a high basal kinase activity. Acts as a mediator of the suppressive effects of IFNgamma on hematopoiesis. Negative regulator for signals that control generation of arsenic trioxide As(2)O(3)-dependent apoptosis and anti-leukemic responses. Involved in anti-apoptotic signaling in response to serum withdrawal. {ECO:0000269|PubMed:11154262, ECO:0000269|PubMed:17689282, ECO:0000269|PubMed:17903173, ECO:0000269|PubMed:20574055, ECO:0000269|PubMed:20823271, ECO:0000269|PubMed:20927323, ECO:0000269|PubMed:9155017}.
- 分子量
- 51.6 kDa
- UniProt
- Q8CDB0
- 途径
- MAPK Pathway
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