EGF Protein (AA 29-1038) (His tag)
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- 抗原 See all EGF 蛋白
- EGF (Epidermal Growth Factor (EGF))
- 蛋白类型
- Recombinant
- 产品特性
- AA 29-1038
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This EGF protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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WQTGNCQPGP LERSERSGTC AGPAPFLVFS QGKSISRIDP DGTNHQQLVV DAGISADMDI HYKKERLYWV DVERQVLLRV FLNGTGLEKV CNVERKVSGL AIDWIDDEVL WVDQQNGVIT VTDMTGKNSR VLLSSLKHPS NIAVDPIERL MFWSSEVTGS LHRAHLKGVD VKTLLETGGI SVLTLDVLDK RLFWVQDSGE GSHAYIHSCD YEGGSVRLIR HQARHSLSSM AFFGDRIFYS VLKSKAIWIA NKHTGKDTVR INLHPSFVTP GKLMVVHPRA QPRTEDAAKD PDPELLKQRG RPCRFGLCER DPKSHSSACA EGYTLSRDRK YCEDVNECAT QNHGCTLGCE NTPGSYHCTC PTGFVLLPDG KQCHELVSCP GNVSKCSHGC VLTSDGPRCI CPAGSVLGRD GKTCTGCSSP DNGGCSQICL PLRPGSWECD CFPGYDLQSD RKSCAASGPQ PLLLFANSQD IRHMHFDGTD YKVLLSRQMG MVFALDYDPV ESKIYFAQTA LKWIERANMD GSQRERLITE GVDTLEGLAL DWIGRRIYWT DSGKSVVGGS DLSGKHHRII IQERISRPRG IAVHPRARRL FWTDVGMSPR IESASLQGSD RVLIASSNLL EPSGITIDYL TDTLYWCDTK RSVIEMANLD GSKRRRLIQN DVGHPFSLAV FEDHLWVSDW AIPSVIRVNK RTGQNRVRLQ GSMLKPSSLV VVHPLAKPGA DPCLYRNGGC EHICQESLGT ARCLCREGFV KAWDGKMCLP QDYPILSGEN ADLSKEVTSL SNSTQAEVPD DDGTESSTLV AEIMVSGMNY EDDCGPGGCG SHARCVSDGE TAECQCLKGF ARDGNLCSDI DECVLARSDC PSTSSRCINT EGGYVCRCSE GYEGDGISCF DIDECQRGAH NCGENAACTN TEGGYNCTCA GRPSSPGLSC PDSTAPSLLG EDGHHLDRNS YPGCPSSYDG YCLNGGVCMH IESLDSYTCN CVIGYSGDRC QTRDLRWWEL RHAGYGQKHD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Egf Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product EGF 蛋白
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Epidermal Growth Factor (EGF) protein
EGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Epidermal Growth Factor (EGF) (Active) proteinEGF 宿主: 人 宿主: 大肠杆菌（E. Coli） > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active
Epidermal Growth Factor (EGF) (AA 971-1023) protein (Fc Tag)EGF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 977-1029) protein (Fc Tag)EGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein (Fc Tag)EGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor (EGF) (Active) proteinEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Epidermal Growth Factor (EGF) (AA 974-1026) protein (His tag)EGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Epidermal Growth Factor (EGF) (AA 977-1029) protein (His-IF2DI Tag)EGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Epidermal Growth Factor (EGF) (AA 926-1025) protein (GST tag)EGF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Epidermal Growth Factor (EGF) (AA 1054-1207) protein (His tag)Crystallography grade EGF 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- EGF (Epidermal Growth Factor (EGF))
- 别名
- Egf (EGF 产品)
- 别名
- HOMG4 Protein, URG Protein, AI790464 Protein, CEGF Protein, epidermal growth factor Protein, pro-epidermal growth factor Protein, EGF Protein, egf Protein, CpipJ_CPIJ020278 Protein, Egf Protein
- 背景
- EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity). {ECO:0000250}.
- 分子量
- 111.8 kDa Including tag.
- UniProt
- P01132
- 途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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