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EGF 蛋白

EGF 宿主: 人 宿主: 大肠杆菌(E. Coli) > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active
产品编号 ABIN987821
发货至: 中国
  • 抗原 See all EGF 蛋白
    EGF (Epidermal Growth Factor (EGF))
    生物活性
    Active
    宿主
    • 31
    • 19
    • 10
    • 4
    • 3
    • 1
    资源
    • 36
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    大肠杆菌(E. Coli)
    序列
    NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW EL
    产品特性
    The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/ml, corresponding to a specific activity of 5.0×105 IU/ mg.
    纯度
    > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
    内毒素水平
    Level Less than 0.1ng/myg (1 IEU/myg) determined by LAL test
    Top Product
    Discover our top product EGF 蛋白
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    It is recommended to reconstitute the lyophilized Recombinant Human Epidermal Growth Factor (EGF) in sterile 18 Mohm-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Quantitation Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. RP-HPLC analysis, using a calibrated solution of EGF as a reference standard.
    储存条件
    -20 °C
  • Li, Niu, Lang, Guo, Ma, Kiesewetter, Backer, Shen, Chen: "PET imaging of EGF receptors using [18F]FBEM-EGF in a head and neck squamous cell carcinoma model." in: European journal of nuclear medicine and molecular imaging, Vol. 39, Issue 2, pp. 300-8, (2012) (PubMed).

  • 抗原
    EGF (Epidermal Growth Factor (EGF))
    Abstract
    EGF 产品
    别名
    HOMG4 Protein, URG Protein, AI790464 Protein, CEGF Protein, epidermal growth factor Protein, pro-epidermal growth factor Protein, EGF Protein, egf Protein, CpipJ_CPIJ020278 Protein, Egf Protein
    背景
    Human Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human Epidermal Growth Factor (EGF) is a 6,200 Da protein containing 53 amino acid residues. Synonym: rHuEGF, Epidermal Growth Facto. Formulation: The protein was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH7.0, 200mM NaCl buffer.
    分子量
    6.0 kDa+/-10% determined by reduced SDS-PAGE Isoelectric Point The main zone between 4.0-5.0 analysis by IEF UV Scan The maximal absorption wave is 275+/-3 nm.
    途径
    NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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