NF-kB p65 Protein (His tag)
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- 抗原 See all NF-kB p65 (NFkBP65) 蛋白
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- Insect cells (Sf21)
- 标记
- This NF-kB p65 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 交叉反应
- 人
- 产品特性
- Human NF-kappaB (p65) is fused to a His-tag.
- 纯度
- >95 % (SDS-PAGE)
- Top Product
- Discover our top product NFkBP65 蛋白
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Nuclear Factor-kB p65 (NFkBP65) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)
NFkBP65 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
Nuclear Factor-kB p65 (NFkBP65) (AA 1-220) protein (GST tag)NFkBP65 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nuclear Factor-kB p65 (NFkBP65) (AA 432-505) protein (GST tag)NFkBP65 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nuclear Factor-kB p65 (NFkBP65) (AA 1-551) protein (Strep Tag)Crystallography grade NFkBP65 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Nuclear Factor-kB p65 (NFkBP65) (AA 1-549) protein (Strep Tag)Crystallography grade NFkBP65 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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~1ng is required for high mobility shift assay.~5ng are required for reconstituted transcription assays.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- In 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride, 0.2 % NP-40, 50 mM imidazole and 10 % glycerol.
- 储存条件
- -20 °C,-80 °C
- 储存方法
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Short Term Storage: -20°C
Long Term Storage: -80°C
Stable for at least 6 months after receipt when stored at -80°C. - 有效期
- 6 months
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- 抗原
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
- 别名
- NF-kB p65 (NFkBP65 产品)
- 别名
- C-Rel Protein, NFKB3 Protein, p65 Protein, NFkB Protein, c-rel Protein, zgc:100833 Protein, Xrel2 Protein, Xrel3 Protein, rel Protein, rel-A Protein, rel2 Protein, rel3 Protein, v-rel Protein, xrel Protein, REL proto-oncogene, NF-kB subunit Protein, RELA proto-oncogene, NF-kB subunit Protein, v-rel reticuloendotheliosis viral oncogene homolog A (avian) Protein, v-rel avian reticuloendotheliosis viral oncogene homolog Protein, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog Protein, REL Protein, RELA Protein, Rela Protein, Rel Protein, rel Protein, rel.L Protein
- 背景
- NF-kappaB is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappaB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric p65-p50 complex is the most abundant complex. The dimers bind at kappaB sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappaB sites that they can bind with distinguishable affinity and specificity.Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappaB complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappaB inhibitor (I-kappaB) family. In a conventional activation pathway, I-kappaB is phosphorylated by I-kappaB kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappaB complex which translocates to the nucleus. NF-kappaB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappaB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappaB complex.
- UniProt
- Q04206
- 途径
- NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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