PRKACA Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- 抗原 See all PRKACA 蛋白
- PRKACA (Protein Kinase A, alpha (PRKACA))
- 蛋白类型
- Recombinant
- 产品特性
- Transcript Variant 1
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This PRKACA protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human PRKACA (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PRKACA 蛋白
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Protein Kinase A, alpha (PRKACA) (AA 1-351) protein (GST tag)
PRKACA 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Protein Kinase A, alpha (PRKACA) (AA 1-120) protein (GST tag)PRKACA 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Protein Kinase A, alpha (PRKACA) (AA 252-350) protein (GST tag)PRKACA 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Protein Kinase A, alpha (PRKACA) (AA 2-351) protein (His tag)Crystallography grade PRKACA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Protein Kinase A, alpha (PRKACA) (AA 2-351) protein (His tag)Crystallography grade PRKACA 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Protein Kinase A, alpha (PRKACA) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)PRKACA 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Protein Kinase A, alpha (PRKACA) (AA 1-351) protein (GST tag)PRKACA 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 85 % by SDS - PAGE SDS
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- PRKACA (Protein Kinase A, alpha (PRKACA))
- 别名
- Prkaca (PRKACA 产品)
- 别名
- PKACA Protein, Calpha Protein, Cs-PKA Protein, PKCA1 Protein, PKA Protein, PKCD Protein, Pkaca Protein, zgc:158799 Protein, protein kinase cAMP-activated catalytic subunit alpha Protein, protein kinase, cAMP dependent, catalytic, alpha Protein, cAMP-dependent protein kinase catalytic subunit Protein, protein kinase, cAMP-dependent, catalytic, alpha, genome duplicate a Protein, PRKACA Protein, Prkaca Protein, kin-1 Protein, prkacaa Protein
- 背景
- This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing&aposs syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.
- 分子量
- 40.4 kDa
- NCBI登录号
- NP_002721
- 途径
- NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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