PDGFB Protein (Transcript Variant 1)
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- 抗原 See all PDGFB 蛋白
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 1
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human PDGFB (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- 内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells.The expected ED50 for this effect is 1.0-3.0 ng/ml.
- Top Product
- Discover our top product PDGFB 蛋白
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Platelet Derived Growth Factor Subunit B (PDGFB) (Active) protein
PDGFB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Platelet Derived Growth Factor Subunit B (PDGFB) protein (His tag)PDGFB 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >85 % as determined by reducing SDS-PAGE.
Platelet Derived Growth Factor Subunit B (PDGFB) (Active) proteinPDGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) protein (GST tag)PDGFB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) protein (His tag)PDGFB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) protein (His tag)Crystallography grade PDGFB 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) protein (His tag)Crystallography grade PDGFB 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) (Active) protein (His tag,AVI tag)PDGFB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE. Active
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
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- 缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- 注意事项
- Resuspend the protein in the desired concentration in proper buffer
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
- 别名
- Pdgfb (PDGFB 产品)
- 别名
- PDGF-2 Protein, PDGF2 Protein, SIS Protein, SSV Protein, c-sis Protein, pdgf2 Protein, sis Protein, si:ch211-79m20.1 Protein, si:dkey-261d11.1 Protein, PDGF-B Protein, Sis Protein, platelet derived growth factor subunit B Protein, platelet-derived growth factor beta polypeptide Protein, platelet derived growth factor subunit B S homeolog Protein, platelet-derived growth factor beta polypeptide a Protein, platelet derived growth factor, B polypeptide Protein, PDGFB Protein, pdgfb.S Protein, pdgfba Protein, Pdgfb Protein
- 背景
- This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants.
- 分子量
- 24.3 kDa
- NCBI登录号
- NP_002599
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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