Epigen 蛋白
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- 抗原 See all Epigen (EPGN) 蛋白
- Epigen (EPGN) (Epithelial Mitogen (EPGN))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human Epithelial mitogen homolog (mouse) (EPGN). protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- 内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells. The expected ED50 for this effect is 150-300 ng/ml.
- Top Product
- Discover our top product EPGN 蛋白
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Epithelial Mitogen (EPGN) (AA 24-95) protein (His tag)
EPGN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Epithelial Mitogen (EPGN) (AA 1-133) protein (GST tag)EPGN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Epithelial Mitogen (EPGN) (AA 23-110) protein (His tag)EPGN 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS
Epithelial Mitogen (EPGN) protein (Myc-DYKDDDDK Tag)EPGN 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
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- 缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- 注意事项
- Resuspend the protein in the desired concentration in proper buffer
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- Epigen (EPGN) (Epithelial Mitogen (EPGN))
- 别名
- Epithelial Mitogen Homolog (Mouse) (Epgn) (EPGN 产品)
- 别名
- ALGV3072 Protein, EPG Protein, PRO9904 Protein, 2310069M11Rik Protein, epigen Protein, RGD1560084 Protein, epithelial mitogen Protein, EPGN Protein, Epgn Protein
- 背景
- The protein encoded by this gene is a member of the epidermal growth factor family. Members of this family are ligands for the epidermal growth factor receptor and play a role in cell survival, proliferation and migration. This protein has been reported to have high mitogenic activity but low affinity for its receptor. Expression of this transcript and protein have been reported in cancer specimens of the breast, bladder, and prostate. Alternative splicing results in multiple transcript variants.
- 分子量
- 7.9 kDa
- NCBI登录号
- NP_001013460
- 途径
- RTK signaling, EGFR Signaling Pathway
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