CHEK2 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- 抗原 See all CHEK2 蛋白
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 1
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CHEK2 protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human CHK2 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- CHEK2 activity verified in a biochemical assay:,CHEK2 activity verified in a biochemical assay:
- Top Product
- Discover our top product CHEK2 蛋白
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Checkpoint Kinase 2 (CHEK2) (AA 1-543) protein (GST tag)
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Checkpoint Kinase 2 (CHEK2) protein (GST tag)CHEK2 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Checkpoint Kinase 2 (CHEK2) protein (GST tag,His tag)CHEK2 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
Checkpoint Kinase 2 (CHEK2) (AA 1-543) protein (Strep Tag)Crystallography grade CHEK2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Checkpoint Kinase 2 (CHEK2) (AA 1-546) protein (Strep Tag)Crystallography grade CHEK2 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- > 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- 别名
- Chk2 (CHEK2 产品)
- 别名
- CDS1 Protein, CHK2 Protein, HuCds1 Protein, LFS2 Protein, PP1425 Protein, RAD53 Protein, hCds1 Protein, fa66f08 Protein, wu:fa66f08 Protein, zgc:55865 Protein, Cds1 Protein, HUCDS1 Protein, Rad53 Protein, Chk2 Protein, cds1 Protein, chek2 Protein, chk2 Protein, hucds1 Protein, lfs2 Protein, pp1425 Protein, rad53 Protein, checkpoint kinase 2 Protein, serine/threonine-protein kinase chk2 Protein, checkpoint kinase 2 L homeolog Protein, CHEK2 Protein, chek2 Protein, Chek2 Protein, MCYG_07308 Protein, chek2.L Protein
- 背景
- In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
- 分子量
- 60.7 kDa
- NCBI登录号
- NP_009125
- 途径
- p53 Pathway, Apoptosis, Cell Division Cycle
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