Quick Overview for p53 Protein (full length) (ABIN2669408)
抗原
See all p53 (TP53) 蛋白蛋白类型
宿主
资源
应用范围
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产品特性
- full length
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产品特性
- Full-length recombinant p53 protein was expressed in a baculovirus system with a polyhistidine tag and purified by an affinity column in combination with FPLC chromatography. Active Motif also offers Recombinant p53 protein (Catalog No. 31465) that was expressed in E. coli.
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纯化方法
- Purified by an affinity column in combination with FPLC chromatography.
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Tumor Protein P53 (TP53) (AA 1-393) protein (His tag)
custom-made TP53 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Protein P53 (TP53) (AA 1-393) protein (GST tag)TP53 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Tumor Protein P53 (TP53) (Active) protein (His tag)TP53 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant STD, PI, in vitro Active
Tumor Protein P53 (TP53) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)TP53 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining STD, AbP
Tumor Protein P53 (TP53) (AA 1-391) protein (His tag)TP53 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Tumor Protein P53 (TP53) protein (His-SUMO Tag)TP53 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90% as determined by SDS-PAGE. ELISA
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应用备注
- Recombinant p53 is suitable for TransAM®, protein-protein and DNA protein assays. 1 ng is sufficient for a gelshift assay. The standard curve for TransAM p53 was generated using the range of 40 - 0.625 ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
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限制
- 仅限研究用
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浓度
- 0.5 μg/μL
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注意事项
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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储存条件
- -20 °C/-80 °C
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储存方法
- Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- p53 (TP53) (Tumor Protein P53 (TP53))
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别名
- p53
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背景
- P53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFκB (NFκB p50 and NFκB p65), both of which serve to inhibit apoptosis.
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途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
抗原
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