GDNF Protein (AA 78-211, N-Term)
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- 抗原 See all GDNF 蛋白
- GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- N-Term, AA 78-211
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- >98 % , as determined by Coomassie stained SDS-PAGE.
- 内毒素水平
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Less than 0.1 ng per μg of protein.
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- Discover our top product GDNF 蛋白
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Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein
GDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein (His tag)GDNF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-177) protein (GST tag)GDNF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 1-211) protein (GST tag)GDNF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein (His tag)Crystallography grade GDNF 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein (His tag)Crystallography grade GDNF 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: ED50 ≤0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg, as measured by its ability to stimulate proliferation of rat C6 cells in a dose dependent manner.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store in working aliquots at -20 °C to -80 °C.
- 缓冲液
- Lyophilized, carrier-free.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- 抗原
- GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
- 别名
- GDNF (GDNF 产品)
- 别名
- gdnf Protein, GDNF Protein, AI385739 Protein, gdnfa Protein, hfb1-gdnf Protein, ATF1 Protein, ATF2 Protein, HFB1-GDNF Protein, HSCR3 Protein, glial cell derived neurotrophic factor Protein, glial cell derived neurotrophic factor a Protein, glial cell line derived neurotrophic factor Protein, glial cell derived neurotrophic factor L homeolog Protein, Gdnf Protein, gdnfa Protein, GDNF Protein, gdnf.L Protein
- 背景
- Glial cell line-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily and was first identified as a growth factor supporting the survival of embryonic midbrain neurons. GDNF has been shown to promote the development, differentiation, and survival of dopaminergic neurons by signaling through GDNF family receptor α-1 (GFRα1) and the coreceptor Ret receptor tyrosine kinase. Dysregulated expression of GDNF has been reported to be associated with several mental diseases, including depression and schizophrenia. GDNF is considered as a promising therapeutic agent for the treatment of Parkinson's disease, since GNDF can protect dopaminergic neurons from neurotoxin and promotes neuron regeneration after injury. In addition to the functions in the nervous system, GDNF is also required for the differentiation of spermatogonia and development of kidney. Upregulated expression of GNDF was observed in various cancer cell lines and correlates with malignant cancer progression, suggesting a role of GDNF in carcinogenesis.
- 分子量
- The 135 amino acid recombinant protein has a predicted molecular mass of approximately 15.0 kDa. Recombinant human GDNF forms a a disulfide-linked homodimer. The predicted N-terminal amino acid is Met.
- 途径
- RTK signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
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