CTLA4 Protein (AA 37-160) (His tag,AVI tag,Biotin)
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- 抗原 See all CTLA4 蛋白
- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 37-160
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宿主
- Cynomolgus, 猕猴
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资源
- HEK-293 Cells
- 标记
- This CTLA4 protein is labelled with His tag,AVI tag,Biotin.
- 品牌
- MABSol®,PrecisionAvi
- 序列
- AA 37-160
- 特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- 产品特性
- This protein carries a Avi tag (AvitagTM) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 15.9 kDa. The protein migrates as 23-26 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
- 纯度
- >95 % as determined by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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- Discover our top product CTLA4 蛋白
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- 说明
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 注意事项
- Please avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
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- 抗原
- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
- 别名
- CTLA-4 (CTLA4 产品)
- 别名
- CTLA-4 Protein, CTLA4 Protein, CD152 Protein, Cd152 Protein, Ctla-4 Protein, Ly-56 Protein, CD Protein, CELIAC3 Protein, GRD4 Protein, GSE Protein, IDDM12 Protein, sCTLA4 Protein, cytotoxic T-lymphocyte associated protein 4 Protein, cytotoxic T-lymphocyte-associated protein 4 Protein, CTLA4 Protein, Ctla4 Protein
- 背景
- CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 Molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
- 分子量
- 15.9 kDa
- NCBI登录号
- XP_005574071
- UniProt
- G7PL88
- 途径
- Cancer Immune Checkpoints
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