Recombinant Human Insulin-Like Growth Factor-Binding Protein 5/IGFBP-5 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Leu21-Glu272) of Human IGFBP5 fused with a 6His tag at the C-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
4 °C/-20 °C/-80 °C
储存方法
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
抗原
IGFBP5
(Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
别名
Insulin-Like Growth Factor-Binding Protein 5/IGFBP-5 (IGFBP5 产品)
别名
IBP5 Protein, AI256729 Protein, AW208790 Protein, IGFBP-5 Protein, IGFBP-5P Protein, IGF-BP5 Protein, igfbp5 Protein, ibp5 Protein, IBP-5 Protein, xIGFBP-5 Protein, IGFBP5 Protein, igfbp5b Protein, zgc:165472 Protein, insulin like growth factor binding protein 5 Protein, insulin-like growth factor binding protein 5 Protein, insulin like growth factor binding protein 5 L homeolog Protein, insulin-like growth factor binding protein 5b Protein, insulin-like growth factor-binding protein 5 Protein, insulin-like growth factor binding protein 5a Protein, IGFBP5 Protein, Igfbp5 Protein, igfbp5.L Protein, igfbp5b Protein, igfbp5 Protein, LOC100194500 Protein, igfbp5a Protein
背景
Insulin-Like Growth Factor-Binding Protein 5 (IGFBP-5) is a secreted protein that belongs to the insulin-like growth factor (IGF) binding proteins superfamily. Members of this family prolong the half-life of the IGFs. They have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGFBP-5 contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP-5 is expressed by fibroblasts, myoblasts and Osteosarcoma. It is also present at lower levels in liver, kidney, and brain. Synonyms: Insulin-Like Growth Factor-Binding Protein 5, IBP-5, IGF-Binding Protein 5, IGFBP-5, IGFBP5, IBP5