Interferon gamma Protein (IFNG)
(3 references)-
- 抗原 See all Interferon gamma (IFNG) 蛋白
- Interferon gamma (IFNG)
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- ELISA, Functional Studies (Func)
- 品牌
- BD Pharmingen™
- 特异性
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Biological Activity: The activity range is measured in an anti-viral assay using A549 indicator cells
Specific Activity: 0.1 - 2.0 x 10^8 Units/mg (Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.)
ED50: 0.05 - 1.0 ng/ml
Observed dose response relationship: 16 fold Since applications vary, each investigator should titrate the reagent to obtain optimal results. - 产品特性
- Recombinant human IFN-gamma is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing 5.0 mg/ml Biotechnology grade, low endotoxin bovine serum albumin, with no azide. Human IFN-gamma was found to be ≥ 95% pure by SDS-PAGE analysis and an absorbance assay based on the Beers-Lambert law.
- 纯化方法
- Recombinant human IFN-γ is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing bovine serum albumin, with no preservatives. Human IFN-γ is ≥ 95 % pure as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law.
- 纯度
- ≥ 95 %
- 过滤
- 0.22 μm filtered
- 内毒素水平
- The endotoxin level is ≤ 0.1 ng/µg of human IFN-gamma, as measured in a chromogenic LAL assay.
- Top Product
- Discover our top product IFNG 蛋白
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Interferon gamma (IFNG) protein (His-Avi Tag)
IFNG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Interferon gamma (IFNG) protein (His-Avi Tag,Biotin)IFNG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
Interferon gamma (IFNG) (Active) proteinIFNG 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interferon gamma (IFNG) (AA 23-155) (Active) proteinIFNG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as analyzed by reducing SDS-PAGE. Active
Interferon gamma (IFNG) (Active) proteinIFNG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as analyzed by reducing SDS-PAGE. Active
Interferon gamma (IFNG) proteinIFNG 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interferon gamma (IFNG) (AA 24-166) protein (His tag)IFNG 宿主: Pig 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interferon gamma (IFNG) (AA 1-144) protein (His tag)IFNG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interferon gamma (IFNG) (AA 24-174) protein (His tag)IFNG 宿主: Hamster 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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- 应用备注
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ELISA Standard: Recombinant human IFN-gamma is useful as a quantitative standard for measuring human IFN-gamma protein levels in a sandwich ELISA with purified NIB42 (Cat. No. 551221) as a capture antibody and the biotinylated 4S.B3 (Cat. No. 554550) as the detection antibody.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring IFN-gamma in serum or plasma our BD OptEIA™ Human IFN-gamma Set (Cat. No. 555142 ) or OptEIA™ Human IFN-gamma Kit (Cat. No. 550612 ) are specially formulated and recommended. - 说明
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BD Pharmingen™ Recombinant Human IFN-γ - Reactivity Hu
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 500 μg/mL
- 缓冲液
- Frozen aqueous buffered solution containing BSA.
- 储存液
- Azide free
- 注意事项
- Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells. IFN-γ exerts its biological effects through specific binding to a single class of high affinity receptors. In addition to its antiviral effects, IFN-γ can upregulate a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. In addition, IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve IFN-γ's capacity to boost MHC class I and II expression by antigen-presenting cells as well as to direct effects on B cells and T cells themselves. Human IFN-γ is a 14 - 18 kD multiple glycosylated protein containing 143 amino acid residues.
- 储存条件
- -80 °C
- 储存方法
- Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
: "The molecular cell biology of interferon-gamma and its receptor." in: Annual review of immunology, Vol. 11, pp. 571-611, (1993) (PubMed).
: "Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons." in: Journal of clinical microbiology, Vol. 12, Issue 3, pp. 433-8, (1981) (PubMed).
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: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
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- 抗原
- Interferon gamma (IFNG)
- 别名
- IFN-gamma (IFNG 产品)
- 别名
- IFG Protein, IFI Protein, IFN-g Protein, Ifg Protein, IFNG2 Protein, IFN-gamma Protein, IFN-G Protein, IFNG Protein, IFNgamma Protein, TCRalpha Protein, INF-G Protein, ifng Protein, interferon gamma Protein, interferon, gamma 1-2 Protein, IFNG Protein, Ifng Protein, ifng1-2 Protein
- 背景
- Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells. IFN-γ exerts its biological effects through specific binding to a single class of high affinity receptors. In addition to its antiviral effects, IFN-γ can upregulate a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. In addition, IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve IFN-γ's capacity to boost MHC class I and II expression by antigen-presenting cells as well as to direct effects on B cells and T cells themselves. Human IFN-γ is a 14 - 18 kD multiple glycosylated protein containing 143 amino acid residues.
- 途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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