This Interferon gamma protein is labelled with His-Avi Tag,Biotin.
序列
Gln24-Gly161
纯度
> 95% as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IFNGR1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IFN gamma, His Tag with the EC50 of 39.1g/ml determined by ELISA. See testing image for detail.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Interferon-gamma, Interferon-γ, interferon, gamma, IFG, IFI, IFN gamma,Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.
分子量
19.1 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.