Osteopontin Protein (AA 17-314) (His tag)
-
- 抗原 See all Osteopontin (SPP1) 蛋白
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 17-314
-
宿主
- 人
-
资源
- 人细胞
- 标记
- This Osteopontin protein is labelled with His tag.
- 原理
- Recombinant Human Secreted Phosphoprotein 1/SPP1 /SPP1 (C-6His)
- 序列
- IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVNVD HHHHHH
- 产品特性
- Recombinant Human Secreted Phosphoprotein 1/SPP1 produced by transfected human cells is a secreted protein with sequence (Ile17-Asn314) of Human SPP1 fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product SPP1 蛋白
-
Secreted phosphoprotein 1 (SPP1) (AA 17-300) protein (His tag)
SPP1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted phosphoprotein 1 (SPP1) (AA 17-294) protein (His tag)SPP1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted phosphoprotein 1 (SPP1) (AA 28-61) protein (His-IF2DI Tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Secreted phosphoprotein 1 (SPP1) (AA 1-300) protein (GST tag)SPP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Secreted phosphoprotein 1 (SPP1) (AA 148-257) protein (GST tag)SPP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Secreted phosphoprotein 1 (SPP1) (AA 21-129) protein (GST tag)SPP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Secreted phosphoprotein 1 (SPP1) proteinSPP1 宿主: 人 Native > 90 % (SDS-PAGE)
Secreted phosphoprotein 1 (SPP1) (AA 17-287) (Active) protein (His tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active
Secreted phosphoprotein 1 (SPP1) (AA 17-300), (partial) protein (His tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Secreted phosphoprotein 1 (SPP1) (AA 17-317) (Active) protein (His tag)SPP1 宿主: 大鼠 宿主: HEK-293 Cells > 95 % AcA, CC Active
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
-
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
-
- 抗原
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- 别名
- spp-1 (SPP1 产品)
- 别名
- BNSP Protein, BSPI Protein, ETA-1 Protein, OPN Protein, 2AR Protein, Apl-1 Protein, Bsp Protein, Eta Protein, OP Protein, Opn Protein, Opnl Protein, Ric Protein, Spp-1 Protein, OSP Protein, zgc:111821 Protein, secreted phosphoprotein 1 Protein, SPP1 Protein, Spp1 Protein, spp1 Protein
- Sub Type
- Fusionprotein
- 背景
-
Secreted Phosphoprotein 1 (SPP1) is a secreted multifunctional glycoprotein. Its putative functions include roles in bone metabolism, immune regulation, wound healing, cell survival, and tumor progression. Based on gene structure and chromosomal location, SPP1 is a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family that also includes bone sialoprotein (BSP), dentin matrix protein 1 (DMP1), dentin sialophosphoprotein (DSPP), enamelin (ENAM), and matrix extracellular phosphoglycoprotein (MEPE). SPP1 is expressed in bone, although it is also expressed in other tissues. SPP1 acts as a cytokine that is involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. It is essential in the pathway that leads to type I immunity. Osteopontin has been implicated as an important factor in bone remodeling. Specifically, research suggests it plays a role in anchoring osteoclasts to the mineral matrix of bones. The fact that SPP1 interacts with multiple cell surface receptors which are ubiquitously expressed makes it an active player in many physiological and pathological processes including wound healing, bone turnover, tumorigenesis, inflammation and ischemia. Therefore, manipulation of plasma Osteopontin levels may be useful in the treatment of autoimmune diseases, cancer metastasis, osteoporosis and some forms of stress.
Alternative Names: Osteopontin, Bone Sialoprotein 1, Nephropontin, Secreted Phosphoprotein 1, SPP-1, Urinary Stone Protein, Uropontin, SPP1, BNSP, OPN - 分子量
- 34.75 kDa
- UniProt
- P10451
- 途径
- Regulation of Cell Size
-
