Osteopontin Protein (AA 17-294) (His tag)
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- 抗原 See all Osteopontin (SPP1) 蛋白
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 17-294
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This Osteopontin protein is labelled with His tag.
- 原理
- Mouse SPP1/OPN Protein
- 序列
- Leu17-Asn294
- 产品特性
- Recombinant Mouse SPP1/OPN Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu17-Asn294.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Top Product
- Discover our top product SPP1 蛋白
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Secreted phosphoprotein 1 (SPP1) (AA 17-300) protein (His tag)
SPP1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted phosphoprotein 1 (SPP1) (AA 28-61) protein (His-IF2DI Tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Secreted phosphoprotein 1 (SPP1) (AA 1-300) protein (GST tag)SPP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Secreted phosphoprotein 1 (SPP1) (AA 148-257) protein (GST tag)SPP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Secreted phosphoprotein 1 (SPP1) (AA 21-129) protein (GST tag)SPP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Secreted phosphoprotein 1 (SPP1) proteinSPP1 宿主: 人 Native > 90 % (SDS-PAGE)
Secreted phosphoprotein 1 (SPP1) (AA 17-314) protein (His tag)Crystallography grade SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Secreted phosphoprotein 1 (SPP1) (AA 17-287) (Active) protein (His tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active
Secreted phosphoprotein 1 (SPP1) (AA 17-300), (partial) protein (His tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Secreted phosphoprotein 1 (SPP1) (AA 17-317) (Active) protein (His tag)SPP1 宿主: 大鼠 宿主: HEK-293 Cells > 95 % AcA, CC Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- 别名
- SPP1 (SPP1 产品)
- 别名
- BNSP Protein, BSPI Protein, ETA-1 Protein, OPN Protein, 2AR Protein, Apl-1 Protein, Bsp Protein, Eta Protein, OP Protein, Opn Protein, Opnl Protein, Ric Protein, Spp-1 Protein, OSP Protein, zgc:111821 Protein, secreted phosphoprotein 1 Protein, SPP1 Protein, Spp1 Protein, spp1 Protein
- 背景
- Secreted phosphoprotein 1 (SPP1) expression in TAMs isolated from lung adenocarcinoma tissues and PMA-treated THP-1 cells were measured. Macrophage polarization was identified by flow cytometric analysis. Cell migration and apoptosis were assessed by Transwell migration assays and flow cytometric analysis, respectively. SPP1 is highly expressed in tumor tissues and TAMs isolated from patients with an advanced TNM stage, and also in PMA-treated THP-1 cells.
- 分子量
- 31.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
- UniProt
- P10923
- 途径
- Regulation of Cell Size
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