Osteopontin Protein (AA 17-300) (His tag)
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- 抗原 See all Osteopontin (SPP1) 蛋白
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 17-300
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Osteopontin protein is labelled with His tag.
- 原理
- Human SPP1/OPN Protein
- 序列
- Ile17-Asn300
- 产品特性
- Recombinant Human SPP1/OPN Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile17-Asn300.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- 别名
- SPP1 (SPP1 产品)
- 别名
- BNSP Protein, BSPI Protein, ETA-1 Protein, OPN Protein, 2AR Protein, Apl-1 Protein, Bsp Protein, Eta Protein, OP Protein, Opn Protein, Opnl Protein, Ric Protein, Spp-1 Protein, OSP Protein, zgc:111821 Protein, secreted phosphoprotein 1 Protein, SPP1 Protein, Spp1 Protein, spp1 Protein
- 背景
- Ovarian cancer is one of the most lethal malignant tumors in women. Secreted phosphoprotein 1 (SPP1) plays an important role in some cancer types. The expression of SPP1 was higher in epithelial ovarian cancer tissues than in normal ovarian tissues. Silencing SPP1 decreased the cell proliferation, migration, and invasion. Ectopic expression of SPP1 increased the cell proliferation, migration, and invasion. Silencing SPP1 prevented ovarian cancer growth in mice. Silencing SPP1 inhibited Integrin β1/FAK/AKT pathway.
- 分子量
- 34.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
- NCBI登录号
- NP_000573
- 途径
- Regulation of Cell Size
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