Osteopontin Protein (AA 17-317) (His tag)
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- 抗原 See all Osteopontin (SPP1) 蛋白
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- 生物活性
- Active
- 产品特性
- AA 17-317
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宿主
- 大鼠
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资源
- HEK-293 Cells
- 标记
- This Osteopontin protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 95 %
- Biological Activity Comment
- Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1x105 cells/mL, and treated with or without various concentrations of OPN for 24h and MCP-1 levels in the cell supernatant were determined by ELISA. MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN, the data was shown in Table 1 and Figure 1. Sample Concentration of MCP-1 O.D. value Corrected (cell supernatant of MCF-7 cells) (ng/mL) stimulated with OPN (100ng/mL) 1.358 1.305 2.53 stimulated with OPN (200ng/mL) 1.177 1.124 2.15 stimulated with OPN (400ng/mL) 1.101 1.048 1.99 unstimulated 0.944 0.891 1.66 Table 1. MCP-1 levels in the cell supernatant of MCF-7 cells regulated by OPN MCP-1 levels in the cell supernatant of MCF-7 cells regulated by OPN.
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- 应用备注
- Isoelectric Point: 4.3
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- 别名
- Osteopontin (OPN) (SPP1 产品)
- 别名
- BNSP Protein, BSPI Protein, ETA-1 Protein, OPN Protein, 2AR Protein, Apl-1 Protein, Bsp Protein, Eta Protein, OP Protein, Opn Protein, Opnl Protein, Ric Protein, Spp-1 Protein, OSP Protein, zgc:111821 Protein, secreted phosphoprotein 1 Protein, SPP1 Protein, Spp1 Protein, spp1 Protein
- 背景
- Alternative Names: SPP1, BNSP, BSPI, ETA1, Secreted Phosphoprotein 1, Bone Sialoprotein I, Early T-Lymphocyte Activation 1, Nephropontin, Urinary stone protein, Uropontin
- 分子量
- 60kDa
- UniProt
- P10451
- 途径
- Regulation of Cell Size
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