anti-猪 Insulin 抗体 for Immunoassay

Recommended Insulin Antibody (supplied by: Log in to see )

抗原
Insulin (INS) 抗体
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
适用

950
468
369
350
239
194
45
11
9
8
7
7
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
宿主
豚鼠
784
196
48
9
4
2
1
克隆类型
多克隆
标记
This Insulin antibody is conjugated to FITC
52
41
35
21
20
20
20
15
15
15
13
13
13
13
13
13
13
10
9
9
7
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
应用范围
Immunoassay (IA)
459
357
275
267
258
237
181
118
106
67
66
31
25
23
21
8
7
5
4
4
4
3
2
2
2
1
1
1
1
1
选项
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产品编号 ABIN458552
$ 537.36
加上运输费 $45.00

General

抗原 Insulin (INS) 抗体
适用
(950), (468), (369), (350), (239), (194), (45), (11), (9), (8), (7), (7), (6), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
宿主 豚鼠
(784), (196), (48), (9), (4), (2), (1)
克隆类型
多克隆
标记 This Insulin antibody is conjugated to FITC
(52), (41), (35), (21), (20), (20), (20), (15), (15), (15), (13), (13), (13), (13), (13), (13), (13), (10), (9), (9), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1)
应用范围 Immunoassay (IA)
(459), (357), (275), (267), (258), (237), (181), (118), (106), (67), (66), (31), (25), (23), (21), (8), (7), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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Insulin抗体详情

Insulin目标详情 使用细节 贮存及处理 图像
特异性 Inter-species cross-reactivity is a normal feature of antibodies to mammalian proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has not been tested for cross-reactivity.
产品特性 Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal guinea pig antiserum to swine insulin
纯化方法 Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG (7S) fraction by DEAE-chromatography.
免疫原 Insulin is a hormone produced by the beta cells of the islets of Langerhans in the pancreas. It plays an important role in the metabolism of carbohydrate by its effects on the storage of glycogen in the liver, the conversion of carbohydrate to fat, and the oxidation of glucose by the peripheral tissues. Insulin has a molecular weight of 6,000 and contains two peptide chains linked by two disulphide bonds. The hormone is isolated from porcine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.
亚型 IgG
Plasmids, Primers & others

Insulin目标详情

Insulin抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 Insulin (INS Antibody 摘要)
背景 The reactivity of the antiserum is directed to insulin as tested by ELISA and immunoblotting. No reaction was obtained with other swine serum proteins.
途径 NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

使用细节

Insulin抗体详情 Insulin目标详情 贮存及处理 图像 回页首
应用备注 The immunoconjugate is intended for use in different types of highly sensitive fluorescence immunoassays on appropriately treated cell and tissue substrates. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions are usually between 1:10 and 1:40.
限制 仅限研究用

贮存及处理

Insulin抗体详情 Insulin目标详情 使用细节 图像 回页首
状态 Lyophilized
浓度 IgG protein concentration 10 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) approximately 2.7. No foreign proteins added.
缓冲液 Purified hyperimmune guinea pig IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
储存液 Without preservative
储存条件 4 °C/-20 °C
储存方法 The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.