anti-牛 MAPK3 抗体 for Immunofluorescence

Recommended MAPK3 Antibody (supplied by: Log in to see )

抗原
Mitogen-Activated Protein Kinase 3 (MAPK3) 抗体
  • ERK-1
  • ERK1
  • ERT2
  • HS44KDAP
  • HUMKER1A
  • P44ERK1
  • P44MAPK
  • PRKM3
  • p44-ERK1
  • p44-MAPK
  • Erk-1
  • Erk1
  • Ert2
  • Esrk1
  • Mnk1
  • Mtap2k
  • Prkm3
  • p44
  • p44erk1
  • p44mapk
  • fi06b09
  • wu:fi06b09
  • zERK1
  • Tb08.10J17.940
  • erk6
  • etID309866.18
  • mapk12
  • sapk3
  • wu:fa05c12
  • zgc:101695
  • MAPK1
  • MNK1
  • ATMAPK3
  • ATMPK3
  • T6D9.4
  • mitogen-activated protein kinase 3
  • mitogen-activated protein kinase 3
  • mitogen-activated protein kinase 12a
  • mitogen activated protein kinase 3
  • mitogen-activated serine/threonine-protein kinase
  • MAPK3
  • Mapk3
  • mapk3
  • Tc00.1047053509475.10
  • Tb927.8.3550
  • mapk12a
  • CEK1
  • MPK3
适用
小鸡, 牛, Fruit Fly (Drosophila melanogaster), 人, 小鼠, 大鼠, 绵羊, 非洲爪蟾
563
330
268
52
41
37
37
23
23
23
20
13
12
11
10
10
7
4
3
2
2
2
1
1
1
1
宿主

400
166
21
6
1
克隆类型
多克隆
标记
This MAPK3 antibody is un-conjugated
29
27
25
17
16
13
6
6
6
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
应用范围
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
493
215
203
110
89
69
59
56
39
35
15
13
8
4
3
2
2
2
1
1
选项
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产品编号 ABIN361833
$ 336.60
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2486608 ICC FACS IF IHC WB Atto 633 Rabbit Log in to see Polyclonal 3
1 ABIN2486610 ICC FACS IF IHC WB Atto 680 Rabbit Log in to see Polyclonal 3
1 ABIN2486919 ICC FACS IF IHC WB APC Rabbit Log in to see Polyclonal 3
1 ABIN2486920 ICC FACS IF IHC WB Biotin Rabbit Log in to see Polyclonal 3
1 ABIN2486921 ICC FACS IF IHC WB FITC Rabbit Log in to see Polyclonal 3
1 ABIN2486923 ICC FACS IF IHC WB PE-Atto 594 Rabbit Log in to see Polyclonal 3
1 ABIN2486926 ICC FACS IF IHC WB Streptavidin Rabbit Log in to see Polyclonal 3
1 ABIN2486922 ICC FACS IF IHC WB HRP Rabbit Log in to see Polyclonal 3
1 ABIN2486604 ICC FACS IF IHC WB Atto 390 Rabbit Log in to see Polyclonal 3
1 ABIN2486606 ICC FACS IF IHC WB Atto 565 Rabbit Log in to see Polyclonal 3
1 ABIN2486605 ICC FACS IF IHC WB Atto 488 Rabbit Log in to see Polyclonal 3
1 ABIN2486925 ICC FACS IF IHC WB PE Rabbit Log in to see Polyclonal 3
1 ABIN2486607 ICC FACS IF IHC WB Atto 594 Rabbit Log in to see Polyclonal 3
1 ABIN2486609 ICC FACS IF IHC WB Atto 655 Rabbit Log in to see Polyclonal 3
1 ABIN2486918 ICC FACS IF IHC WB Alkaline Phosphatase (AP) Rabbit Log in to see Polyclonal 3
1 ABIN2486924 ICC FACS IF IHC WB PerCP Rabbit Log in to see Polyclonal 3
1 ABIN2486611 ICC FACS IF IHC WB Atto 700 Rabbit Log in to see Polyclonal 3
1 ABIN5551547 IF IHC (fro) WB Rabbit IgG Tyr204, pThr202 Log in to see Polyclonal 0
1 ABIN5551325 IF IHC (fro) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0

General

抗原 Mitogen-Activated Protein Kinase 3 (MAPK3) 抗体
适用 小鸡, 牛, Fruit Fly (Drosophila melanogaster), 人, 小鼠, 大鼠, 绵羊, 非洲爪蟾
(563), (330), (268), (52), (41), (37), (37), (23), (23), (23), (20), (13), (12), (11), (10), (10), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
宿主
(400), (166), (21), (6), (1)
克隆类型
多克隆
标记 This MAPK3 antibody is un-conjugated
(29), (27), (25), (17), (16), (13), (6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
应用范围 Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(493), (215), (203), (110), (89), (69), (59), (56), (39), (35), (15), (13), (8), (4), (3), (2), (2), (2), (1), (1)
Pubmed 9个引用可参考
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MAPK3抗体详情

MAPK3目标详情 使用细节 贮存及处理 References for anti-MAPK3 antibody (ABIN361833) 图像
特异性 Detects ~44kda (ERK1) and ~42 kDa (ERK2).
纯化方法 Peptide Affinity Purified
免疫原 A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
Plasmids, Primers & others

MAPK3目标详情

MAPK3抗体详情 使用细节 贮存及处理 References for anti-MAPK3 antibody (ABIN361833) 图像 回页首
抗原
别名 ERK1 (MAPK3 Antibody 摘要)
背景 The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5), however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).
基因ID 50689
NCBI登录号 NP_059043
UniProt P21708
途径 MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

使用细节

MAPK3抗体详情 MAPK3目标详情 贮存及处理 References for anti-MAPK3 antibody (ABIN361833) 图像 回页首
应用备注
  • WB (1:1000)
  • IHC (1:100)
  • ICC/IF (1:100)
  • FCM (1:100)
  • optimal dilutions for assays should be determined by the user.
说明

A 1:1000 dilution of SPC-120 was sufficient for detection of ERK1/2 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

限制 仅限研究用

贮存及处理

MAPK3抗体详情 MAPK3目标详情 使用细节 References for anti-MAPK3 antibody (ABIN361833) 图像 回页首
状态 Liquid
浓度 1 mg/mL
缓冲液 PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
储存条件 -20 °C

References for anti-MAPK3 antibody (ABIN361833)

MAPK3抗体详情 MAPK3目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Revuelta-López, Cal, Herraiz-Martínez, de Gonzalo-Calvo, Nasarre, Roura, Gálvez-Montón, Bayes-Genis, Badimon, Hove-Madsen, Llorente-Cortés: "Hypoxia-driven sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) downregulation depends on low-density lipoprotein receptor-related protein 1 (LRP1)-signalling in cardiomyocytes." in: Journal of molecular and cellular cardiology, Vol. 85, pp. 25-36, 2015

Thon, Hosoi, Yoshii, Ozawa: "Leptin induced GRP78 expression through the PI3K-mTOR pathway in neuronal cells." in: Scientific reports, Vol. 4, pp. 7096, 2014

Bhat, Gomis, Potter, Tikoo: "Role of Hsp90 in CpG ODN mediated immunostimulation in avian macrophages." in: Molecular immunology, Vol. 47, Issue 6, pp. 1337-46, 2010

Enikanolaiye, Larivière, Troy, Arabzadeh, Atasoy, Turksen: "Involucrin-claudin-6 tail deletion mutant (CDelta206) transgenic mice: a model of delayed epidermal permeability barrier formation and repair." in: Disease models & mechanisms, Vol. 3, Issue 3-4, pp. 167-80, 2010

背景

Chuderland, Marmor, Shainskaya, Seger: "Calcium-mediated interactions regulate the subcellular localization of extracellular signal-regulated kinases." in: The Journal of biological chemistry, Vol. 283, Issue 17, pp. 11176-88, 2008

Yoon, Seger: "The extracellular signal-regulated kinase: multiple substrates regulate diverse cellular functions." in: Growth factors (Chur, Switzerland), Vol. 24, Issue 1, pp. 21-44, 2006

Zhuang, Schnellmann: "A death-promoting role for extracellular signal-regulated kinase." in: The Journal of pharmacology and experimental therapeutics, Vol. 319, Issue 3, pp. 991-7, 2006

Wolf: "Role of reactive oxygen species in angiotensin II-mediated renal growth, differentiation, and apoptosis." in: Antioxidants & redox signaling, Vol. 7, Issue 9-10, pp. 1337-45, 2005

Boulton, Gregory, Cobb: "Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase." in: Biochemistry, Vol. 30, Issue 1, pp. 278-86, 1991

图像

MAPK3抗体详情 MAPK3目标详情 使用细节 贮存及处理 References for anti-MAPK3 antibody (ABIN361833) 回页首
厂商提供的图像
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (ABIN361833) Erk1 2 Western Blotting 1 in 1000 human cell line mix 10ug.