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TNFSF4 ELISA 试剂盒
TNFSF4
适用: 人
Colorimetric
Sandwich ELISA
61-15.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
TNFSF4ELISA Kit详情
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TNFSF4
(Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
适用
All reactivities for TNFSF4 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
61-15.000 pg/mL
最低检测浓度
61 pg/mL
应用范围
ELISA
原理
Human OX40 Ligand (TNFSF4) ELISA Kit for serum, plasma, and cell culture supernatant samples.
样品类型
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
特异性
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human AMIGO, Aminopeptidase LRAP (ERAP2), Amnionless, Arylsulfatase A, Bcl-w, CD109, CD157, CD34, CD83, CLEC-1, CLEC10A (CD301), CMG-2, CREG, Cystatin SN, Cytokeratin-8, Dectin-1, Desmocollin-3, Endoglycan, Galectin-4, HAPLN1, Jagged 1, Langerin CD207), Lumican, Matriptase (ST14), MEP1B, Nectin-3, OX40 (TNFRSF4), p27, Pappalysin-2, Plexin B3, Plexin D1, proGRP, PSA-total, Reg1B, RGM-A, ROBO2, Spinesin, TWEAK R (TNFRSF12), ULBP-3.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Recommended Dilution for serum and plasma samples2 fold
样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
TNFSF4目标详情
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TNFSF4
(Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
别名
OX40 Ligand///TNFSF4 (TNFSF4 产品 )
别名
TNFSF4 ELISA Kit, OX40L ELISA Kit, TNFRSF4 ELISA Kit, CD134L ELISA Kit, CD252 ELISA Kit, GP34 ELISA Kit, OX-40L ELISA Kit, OX4OL ELISA Kit, TXGP1 ELISA Kit, Ath-1 ELISA Kit, Ath1 ELISA Kit, Ox40l ELISA Kit, Txgp1l ELISA Kit, gp34 ELISA Kit, TNF superfamily member 4 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 4 ELISA Kit, TNFSF4 ELISA Kit, Tnfsf4 ELISA Kit
基因ID
7292
UniProt
P23510
途径
Cellular Response to Molecule of Bacterial Origin , Regulation of Leukocyte Mediated Immunity , Positive Regulation of Immune Effector Process , Production of Molecular Mediator of Immune Response , Activated T Cell Proliferation , Cancer Immune Checkpoints
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