TNFSF4 ELISA 试剂盒
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- 抗原 See all TNFSF4 ELISA试剂盒
- TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
- 抗原表位
- AA 51-198
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFSF4/OX40L
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: S51-L198 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: S51-L198 - Featured
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Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) ELISA Kit
TNFSF4 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) ELISA KitTNFSF4 适用: 人 AA 51-183 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse TNFSF4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFSF4 has been precoated onto 96-well plates. Standards(NSO, S51-L198) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFSF4 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse TNFSF4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFSF4 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 534, Standard deviation: 23.5, CV(%): 4.4
- Sample 2: n=16, Mean(pg/ml): 1107, Standard deviation: 43.2, CV(%): 3.9
- Sample 3: n=16, Mean(pg/ml): 2374, Standard deviation: 125.8, CV(%): 5.3,
- Sample 1: n=24, Mean(pg/ml): 639, Standard deviation: 37.1, CV(%): 5.8
- Sample 2: n=24, Mean(pg/ml): 1320, Standard deviation: 60.7, CV(%): 4.6
- Sample 3: n=24, Mean(pg/ml): 2448, Standard deviation: 156.7, CV(%): 6.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all TNFSF4 ELISA试剂盒
- TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
- 别名
- TNFSF4 (TNFSF4 产品)
- 别名
- TNFSF4 ELISA Kit, OX40L ELISA Kit, TNFRSF4 ELISA Kit, CD134L ELISA Kit, CD252 ELISA Kit, GP34 ELISA Kit, OX-40L ELISA Kit, OX4OL ELISA Kit, TXGP1 ELISA Kit, Ath-1 ELISA Kit, Ath1 ELISA Kit, Ox40l ELISA Kit, Txgp1l ELISA Kit, gp34 ELISA Kit, TNF superfamily member 4 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 4 ELISA Kit, TNFSF4 ELISA Kit, Tnfsf4 ELISA Kit
- 背景
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Protein Function: Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
Background: OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, Baum et al.(1994) mapped the mouse Tnfsf4 gene to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene.OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.
Synonyms: Tumor necrosis factor ligand superfamily member 4,OX40 ligand,OX40L,CD252,Tnfsf4,Ox40l, Txgp1l,
Full Gene Name: Tumor necrosis factor ligand superfamily member 4
Cellular Localisation: Membrane, Single-pass type II membrane protein. - 基因ID
- 22164
- UniProt
- P43488
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation, Cancer Immune Checkpoints
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