CCL2 ELISA 试剂盒
-
- 抗原 See all CCL2 ELISA试剂盒
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
-
适用
- 小鼠, 人, 犬
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 9-700 pg/mL
- 最低检测浓度
- 9 pg/mL
- 应用范围
- ELISA
- 原理
- Canine MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects canine MCP 1. Less than 0.05 % cross reactivity with recombinant mouse MCP-1 and recombinant human MCP-1 is observed in this antibody pair.
- 灵敏度
- 9 pg/mL
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling CCL2 ELISA Kit
- Top Product
- Discover our top product CCL2 ELISA Kit
-
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 人 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Saliva, Serum, Tissue Homogenate, Urine
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 小鼠, 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 人 AA 24-99 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 猴 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 小鼠 AA 24-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA KitCCL2 适用: 大鼠 AA 24-148 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
-
- 应用备注
- Recommended Dilution for serum and plasma samples2 - 10 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all CCL2 ELISA试剂盒
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- 别名
- MCP-1 (CCL2 产品)
- 别名
- GDCF-2 ELISA Kit, HC11 ELISA Kit, HSMCR30 ELISA Kit, MCAF ELISA Kit, MCP-1 ELISA Kit, MCP1 ELISA Kit, SCYA2 ELISA Kit, SMC-CF ELISA Kit, AI323594 ELISA Kit, JE ELISA Kit, Scya2 ELISA Kit, Sigje ELISA Kit, MCP-1A ELISA Kit, MCP1A ELISA Kit, C-C motif chemokine ligand 2 ELISA Kit, chemokine (C-C motif) ligand 2 ELISA Kit, C-C motif chemokine 2 ELISA Kit, CCL2 ELISA Kit, Ccl2 ELISA Kit, LOC101120093 ELISA Kit
- 背景
-
Gene Names: CCL2 MCP1 SCYA2
Protein names: C-C motif chemokine 2 (Monocyte chemoattractant protein 1) (Monocyte chemotactic protein 1) (MCP-1) (Small-inducible cytokine A2)
- 基因ID
- 403981
- UniProt
- P52203
- 途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
-
