我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

CCL2 ELISA 试剂盒

Name: CCL2 ELISA 试剂盒
SKU: ABIN1672827
Availability: OutOfStock

The 大鼠 CCL2 ELISA Kit (ABIN1672827) is a Colorimetric ELISA Kit designed to quantify 大鼠 CCL2. This ELISA Kit has been cited in 23 publications.

ELISA

1 image

(23 references)

产品编号 ABIN1672827

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CCL2 ELISA 试剂盒 (ABIN1672827)

抗原

See all CCL2 ELISA试剂盒

CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

抗原表位

AA 24-148

适用

大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum

Discover our top product CCL2 ELISA Kit

最低检测浓度

15.6 pg/mL

原理

Sandwich Rat MCP-1 ELISA Kit to quantitate Rat CCL2 in cell culture supernatants and serum.

Analytical Method

Quantitative

特异性

Expression system for standard: NS0
Immunogen sequence: Q24-N148
Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 10 pg/mL

组件
1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers
试剂未包括

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 人 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Saliva, Serum, Tissue Homogenate, Urine

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 小鼠, 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 人 AA 24-99 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 兔 Colorimetric Sandwich ELISA 23.5 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 猴 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 小鼠 AA 24-96 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

样本量

100 μL

板类型

Pre-coated

实验流程

ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

实验精密度

Intra-Assay Precision (CV%): 7.2%, 7.2%, 5.8%
Inter-Assay Precision (CV%): 6.4%, 8.6%, 5.8%

限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles. (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

有效期

12 months
Zhang, Xu, Hu, Wu, Qin, Zhang: "Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway." in: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1293-1304, (2018) (PubMed).

Mei, Wang, Li, Liu, Lu, Li, Zhang, Tian: "Dusuqing granules (DSQ) suppress inflammation in Klebsiella pneumonia rat via NF-κB/MAPK signaling." in: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 216, (2017) (PubMed).

Liu, Zhang, Han, Wang, Liu, Zhang, Zhou, Xiang: "Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." in: Molecular medicine reports, Vol. 16, Issue 1, pp. 192-200, (2017) (PubMed).

Bi, Bi, Zhong, Zhang, Jin, Ma, Luo: "Effects of leukotriene B4 on interleukin-32, interferon-γ and chemokines in rats with rheumatoid arthritis." in: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 2925-2930, (2017) (PubMed).

Yang, Zhang, Wang, Zhao, Shi: "SOCS2 overexpression alleviates diabetic nephropathy in rats by inhibiting the TLR4/NF-κB pathway." in: Oncotarget, Vol. 8, Issue 53, pp. 91185-91198, (2017) (PubMed).

Chen, Zhang, Ma, Huang, Shao, Wu, Wang: "Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction." in: PLoS ONE, Vol. 10, Issue 11, pp. e0143868, (2016) (PubMed).

Mao, Sun, Mao, Wang, Zhang, Zhou, Rahman, Ye: "Inhibitory Effects of Angelica Polysaccharide on Activation of Mast Cells." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 6063475, (2016) (PubMed).

Tu, Chen, Yang, Qi, Lu, Zhao: "Amyloid-? Activates Microglia and Regulates Protein Expression in a Manner Similar to Prions." in: Journal of molecular neuroscience : MN, Vol. 56, Issue 2, pp. 509-18, (2015) (PubMed).

Jiang, Zhang, Xiong, Jia, Sun, Lin, Shen, Xie, Yan: "EETs Attenuate Ox-LDL-Induced LTB4 Production and Activity by Inhibiting p38 MAPK Phosphorylation and 5-LO/BLT1 Receptor Expression in Rat Pulmonary Arterial Endothelial Cells." in: PLoS ONE, Vol. 10, Issue 6, pp. e0128278, (2015) (PubMed).

Aizman, Vinodkumar, McGrogan, Bates: "Cell Injury-Induced Release of Fibroblast Growth Factor 2: Relevance to Intracerebral Mesenchymal Stromal Cell Transplantations." in: Stem cells and development, Vol. 24, Issue 14, pp. 1623-34, (2015) (PubMed).

Dai, Wu, He, Zhang, Chen, Xu, Wang, Gruzdev, Zeldin, Wang: "Epoxyeicosatrienoic acids regulate macrophage polarization and prevent LPS-induced cardiac dysfunction." in: Journal of cellular physiology, Vol. 230, Issue 9, pp. 2108-19, (2015) (PubMed).

Zhang, Jiang, Chen, Wang, Feng, Tao, Qin: "Serum Levels of IL-1 ? , IL-6, TGF- ? , and MMP-9 in Patients Undergoing Carotid Artery Stenting and Regulation of MMP-9 in a New In Vitro Model of THP-1 Cells Activated by Stenting." in: Mediators of inflammation, Vol. 2015, pp. 956082, (2015) (PubMed).

Wei, Zhang, He, Wang, Liu, Liu, Sun, Zhou, Bai, Yuan et al.: "Protective effect of saponins extract from Panax japonicus on myocardial infarction: involvement of NF-?B, Sirt1 and mitogen-activated protein kinase signalling pathways and inhibition of ..." in: The Journal of pharmacy and pharmacology, Vol. 66, Issue 11, pp. 1641-51, (2014) (PubMed).

Tu, Yang, Zhou, Qi, Wang, Kouadir, Xu, Yin, Zhao: "PrP106-126 and A? 1-42 peptides induce BV-2 microglia chemotaxis and proliferation." in: Journal of molecular neuroscience : MN, Vol. 52, Issue 1, pp. 107-16, (2014) (PubMed).

Wang, Gao, Wang, Zhao, Nie, Shi, Zhao, Lu, Wang, Yang: "Green tea polyphenol epigallocatechin-3-gallate inhibits TNF-?-induced production of monocyte chemoattractant protein-1 in human umbilical vein endothelial cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 33, Issue 5, pp. 1349-58, (2014) (PubMed).

Meneses, Libório, de Daher, da Silva, da Costa, Pontes, Martins: "Urinary monocyte chemotactic protein-1 (MCP-1) in leprosy patients: increased risk for kidney damage." in: BMC infectious diseases, Vol. 14, pp. 451, (2014) (PubMed).

Xu, Kong, Zhou, Zhang, Liu, Yan, Xie, Xie: "oxLDL/?2GPI/anti-?2GPI complex induced macrophage differentiation to foam cell involving TLR4/NF-kappa B signal transduction pathway." in: Thrombosis research, Vol. 134, Issue 2, pp. 384-92, (2014) (PubMed).

Lin, Liu, Fang, Yu, Xiong, Yuan: "Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 68, pp. 290-6, (2014) (PubMed).

Huang, Wang, Liu, Zheng, Li: "Inhibitory effect of Malvidin on TNF-?-induced inflammatory response in endothelial cells." in: European journal of pharmacology, Vol. 723, pp. 67-72, (2014) (PubMed).

Jiang, Zhang, Liu, Lin, Sun, Shen, Yan, Xie: "EETs alleviate ox-LDL-induced inflammation by inhibiting LOX-1 receptor expression in rat pulmonary arterial endothelial cells." in: European journal of pharmacology, Vol. 727, pp. 43-51, (2014) (PubMed).

show more references
抗原 See all CCL2 ELISA试剂盒

CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

别名

CCL2

背景

Background: Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3
Gene Full Name: C-C motif chemokine ligand 2

基因ID

24770

UniProt

P14844

途径

Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection