我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

CCL2 ELISA 试剂盒

Name: CCL2 ELISA 试剂盒
SKU: ABIN411320
Availability: OutOfStock

This Colorimetric ELISA kit is designed for the quantitative measurement of 人 CCL2. There are 33 publications available.

ELISA

1 image

(33 references)

产品编号 ABIN411320

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CCL2 ELISA 试剂盒 (ABIN411320)

抗原

See all CCL2 ELISA试剂盒

CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

抗原表位

AA 24-99

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine

Discover our top product CCL2 ELISA Kit

最低检测浓度

15.6 pg/mL

原理

Sandwich Human MCP-1 / CCL2 ELISA Kit to quantitate Human CCL2 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: Q24-T99
Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

交叉反应（详细）

There is cross-reactivity with human Eotaxin, MCP-3<1 % .

灵敏度

< 1 pg/mL

组件
1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers
试剂未包括

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 人 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Saliva, Serum, Tissue Homogenate, Urine

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 小鼠, 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 兔 Colorimetric Sandwich ELISA 23.5 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 猴 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 小鼠 AA 24-96 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Serum

Chemokine (C-C Motif) Ligand 2 (CCL2) ELISA Kit
CCL2 适用: 大鼠 AA 24-148 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

样本量

100 μL

板类型

Pre-coated

实验流程

ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

实验精密度

Intra-Assay Precision (CV%): 5%, 5.5%, 6.9%
Inter-Assay Precision (CV%): 6.6%, 6.6%, 7.8%

限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

有效期

12 months
Namlı Kalem, Akgun, Kalem, Bakirarar, Celik: "Chemokine (C-C motif) ligand-2 (CCL2) and oxidative stress markers in recurrent pregnancy loss and repeated implantation failure." in: Journal of assisted reproduction and genetics, Vol. 34, Issue 11, pp. 1501-1506, (2018) (PubMed).

Sun, Chen, Wang, Wan, Zhang, Zhang, Lin, Zhang: "Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation of Peroxisome Proliferator-Activated Receptor Gamma." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 6905217, (2018) (PubMed).

Capistrano, Meneses, de Oliveira Neves, de Almeida Leitão, Martins, Libório: "Renal Evaluation in Common Variable Immunodeficiency." in: Journal of immunology research, Vol. 2018, pp. 5841031, (2018) (PubMed).

Blanchard, Stepanovska, Starck, Erhardt, Römer, Meyer Zu Heringdorf, Pfeilschifter, Zangemeister-Wittke, Huwiler: "Downregulation of the S1P Transporter Spinster Homology Protein 2 (Spns2) Exerts an Anti-Fibrotic and Anti-Inflammatory Effect in Human Renal Proximal Tubular Epithelial Cells." in: International journal of molecular sciences, Vol. 19, Issue 5, (2018) (PubMed).

Liao, Cai, Xu, Wang, Qiu, Xie, Huang, Sui: "Protective Role of Antioxidant Huskless Barley Extracts on TNF-α-Induced Endothelial Dysfunction in Human Vascular Endothelial Cells." in: Oxidative medicine and cellular longevity, Vol. 2018, pp. 3846029, (2018) (PubMed).

Bao, Li, Luo, Tang, Lv, Li, Zhou: "NF-κB-Regulated miR-99a Modulates Endothelial Cell Inflammation." in: Mediators of inflammation, Vol. 2016, pp. 5308170, (2017) (PubMed).

Sun, Zhu, Cai, Qiu: "Hypaphorine Attenuates Lipopolysaccharide-Induced Endothelial Inflammation via Regulation of TLR4 and PPAR-γ Dependent on PI3K/Akt/mTOR Signal Pathway." in: International journal of molecular sciences, Vol. 18, Issue 4, (2017) (PubMed).

Chen, Liu, Chen, Tseng, Shen, Hwang, Hsu: "Oligo-Fucoidan prevents IL-6 and CCL2 production and cooperates with p53 to suppress ATM signaling and tumor progression." in: Scientific reports, Vol. 7, Issue 1, pp. 11864, (2017) (PubMed).

Liu, Li, Liu, Wang, Jia: "Inhibition of zymosan-induced cytokine and chemokine expression in human corneal fibroblasts by triptolide." in: International journal of ophthalmology, Vol. 9, Issue 1, pp. 9-14, (2016) (PubMed).

Aizman, Vinodkumar, McGrogan, Bates: "Cell Injury-Induced Release of Fibroblast Growth Factor 2: Relevance to Intracerebral Mesenchymal Stromal Cell Transplantations." in: Stem cells and development, Vol. 24, Issue 14, pp. 1623-34, (2015) (PubMed).

Jiang, Zhang, Xiong, Jia, Sun, Lin, Shen, Xie, Yan: "EETs Attenuate Ox-LDL-Induced LTB4 Production and Activity by Inhibiting p38 MAPK Phosphorylation and 5-LO/BLT1 Receptor Expression in Rat Pulmonary Arterial Endothelial Cells." in: PLoS ONE, Vol. 10, Issue 6, pp. e0128278, (2015) (PubMed).

Tu, Chen, Yang, Qi, Lu, Zhao: "Amyloid-? Activates Microglia and Regulates Protein Expression in a Manner Similar to Prions." in: Journal of molecular neuroscience : MN, Vol. 56, Issue 2, pp. 509-18, (2015) (PubMed).

Zhang, Jiang, Chen, Wang, Feng, Tao, Qin: "Serum Levels of IL-1 ? , IL-6, TGF- ? , and MMP-9 in Patients Undergoing Carotid Artery Stenting and Regulation of MMP-9 in a New In Vitro Model of THP-1 Cells Activated by Stenting." in: Mediators of inflammation, Vol. 2015, pp. 956082, (2015) (PubMed).

Han, Wang, Zhang, Liu, Li, Wang, Song, Pang, Ouyang, Tang: "Apolipoprotein CIII regulates lipoprotein-associated phospholipase A2 expression via the MAPK and NF?B pathways." in: Biology open, Vol. 4, Issue 5, pp. 661-5, (2015) (PubMed).

Dai, Wu, He, Zhang, Chen, Xu, Wang, Gruzdev, Zeldin, Wang: "Epoxyeicosatrienoic acids regulate macrophage polarization and prevent LPS-induced cardiac dysfunction." in: Journal of cellular physiology, Vol. 230, Issue 9, pp. 2108-19, (2015) (PubMed).

Li, Wang, Zhang, Ma, Zhao: "IL-1β mediates MCP-1 induction by Wnt5a in gastric cancer cells." in: BMC cancer, Vol. 14, pp. 480, (2015) (PubMed).

Liu, Wang, Ding, Wang, Zeng: "A potential therapeutic effect of CYP2C8 overexpression on anti-TNF-α activity." in: International journal of molecular medicine, Vol. 34, Issue 3, pp. 725-32, (2015) (PubMed).

Meneses, Libório, de Daher, da Silva, da Costa, Pontes, Martins: "Urinary monocyte chemotactic protein-1 (MCP-1) in leprosy patients: increased risk for kidney damage." in: BMC infectious diseases, Vol. 14, pp. 451, (2014) (PubMed).

Xu, Kong, Zhou, Zhang, Liu, Yan, Xie, Xie: "oxLDL/?2GPI/anti-?2GPI complex induced macrophage differentiation to foam cell involving TLR4/NF-kappa B signal transduction pathway." in: Thrombosis research, Vol. 134, Issue 2, pp. 384-92, (2014) (PubMed).

Tu, Yang, Zhou, Qi, Wang, Kouadir, Xu, Yin, Zhao: "PrP106-126 and A? 1-42 peptides induce BV-2 microglia chemotaxis and proliferation." in: Journal of molecular neuroscience : MN, Vol. 52, Issue 1, pp. 107-16, (2014) (PubMed).

show more references
抗原 See all CCL2 ELISA试剂盒

CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

别名

CCL2

背景

Background: Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.
Gene Full Name: C-C motif chemokine ligand 2

基因ID

6347

UniProt

P13500

途径

Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection