CXCL1 ELISA 试剂盒
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- 抗原 See all CXCL1 ELISA试剂盒
- CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- For quantitative detection of CXCL1 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernates.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 灵敏度
- < 1 pg/mL
- 组件
- 1. One 96-well plate pre-coated with anti-Mouse CXCL1 antibody 2. Lyophilized Mouse CXCL1 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Mouse CXCL1 antibody (Concentrated): 130 µl.
- 试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
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- Discover our best selling CXCL1 ELISA Kit
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- Discover our top product CXCL1 ELISA Kit
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Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 猴 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 小鼠 AA 20-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 大鼠 AA 25-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 人 AA 35-107 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
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- 说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CXCL1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CXCL1 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the CXCL1 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of CXCL1 can be calculated.
- 板类型
- Pre-coated
- 试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- 样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Tissue lysate, body fluids or cell culture supernate: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 2000 × g for 20 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin, citrate or EDTA as the anticoagulant. Centrifuge for 30 min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. - 限制
- 仅限研究用
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- 储存液
- Sodium azide, Thimerosal (Merthiolate)
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- 抗原 See all CXCL1 ELISA试剂盒
- CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
- 别名
- CXCL1 / GRO-alpha (CXCL1 产品)
- 别名
- FSP ELISA Kit, GRO1 ELISA Kit, GROa ELISA Kit, MGSA ELISA Kit, MGSA-a ELISA Kit, NAP-3 ELISA Kit, SCYB1 ELISA Kit, CINC-1 ELISA Kit, Gro1 ELISA Kit, CXCL1 ELISA Kit, Sdf1 ELISA Kit, Fsp ELISA Kit, KC ELISA Kit, Mgsa ELISA Kit, N51 ELISA Kit, Scyb1 ELISA Kit, gro ELISA Kit, GRO ELISA Kit, Gro ELISA Kit, C-X-C motif chemokine ligand 1 ELISA Kit, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) ELISA Kit, C-X-C motif chemokine ligand 12 ELISA Kit, chemokine (C-X-C motif) ligand 1 ELISA Kit, CXCL1 ELISA Kit, Cxcl1 ELISA Kit, Cxcl12 ELISA Kit, GRO1 ELISA Kit
- 背景
- Chemokine (C-X-C motif) ligand 1 (CXCL1), also called GRO1 oncogene, GROĮ, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-Į), is a small cytokine belonging to the CXC chemokine family . In humans, this protein is encoded by the CXCL1 gene. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. It is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis.
- 途径
- Autophagy
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