CXCL1 ELISA 试剂盒
(2 references)-
- 抗原 See all CXCL1 ELISA试剂盒
- CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
- 抗原表位
- AA 35-107
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: E.coli
Immunogen sequence: A35-N107 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <1pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: E.coli
Immunogen sequence: A35-N107 - Featured
- Discover our best selling CXCL1 ELISA Kit
- Top Product
- Discover our top product CXCL1 ELISA Kit
-
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 猴 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 小鼠 AA 20-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 大鼠 AA 25-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA KitCXCL1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.
- 板类型
- Pre-coated
- 实验流程
- human CXCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL1 has been precoated onto 96-well plates. Standards(E. coli, A35-N107) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CXCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL1 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 83.8, Standard deviation: 3.6, CV(%): 4.3
- Sample 2: n=16, Mean(pg/ml): 357, Standard deviation: 12.8, CV(%): 3.6
- Sample 3: n=16, Mean(pg/ml): 723, Standard deviation: 24.6, CV(%): 3.4,
- Sample 1: n=24, Mean(pg/ml): 95.9, Standard deviation: 5.85, CV(%): 6.1
- Sample 2: n=24, Mean(pg/ml): 363, Standard deviation: 18.9, CV(%): 5.2
- Sample 3: n=24, Mean(pg/ml): 716, Standard deviation: 45.82, CV(%): 6.4
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
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: "IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel." in: Nature communications, Vol. 6, pp. 8746, (2016) (PubMed).
: "After insufficient radiofrequency ablation, tumor-associated endothelial cells exhibit enhanced angiogenesis and promote invasiveness of residual hepatocellular carcinoma." in: Journal of translational medicine, Vol. 10, pp. 230, (2013) (PubMed).
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: "IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel." in: Nature communications, Vol. 6, pp. 8746, (2016) (PubMed).
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- 抗原 See all CXCL1 ELISA试剂盒
- CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
- 别名
- CXCL1 (CXCL1 产品)
- 别名
- FSP ELISA Kit, GRO1 ELISA Kit, GROa ELISA Kit, MGSA ELISA Kit, MGSA-a ELISA Kit, NAP-3 ELISA Kit, SCYB1 ELISA Kit, CINC-1 ELISA Kit, Gro1 ELISA Kit, CXCL1 ELISA Kit, Sdf1 ELISA Kit, Fsp ELISA Kit, KC ELISA Kit, Mgsa ELISA Kit, N51 ELISA Kit, Scyb1 ELISA Kit, gro ELISA Kit, GRO ELISA Kit, Gro ELISA Kit, C-X-C motif chemokine ligand 1 ELISA Kit, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) ELISA Kit, C-X-C motif chemokine ligand 12 ELISA Kit, chemokine (C-X-C motif) ligand 1 ELISA Kit, CXCL1 ELISA Kit, Cxcl1 ELISA Kit, Cxcl12 ELISA Kit, GRO1 ELISA Kit
- 背景
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Protein Function: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. .
Background: Chemokine(C-X-C motif) ligand 1(CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3(NAP-3) and melanoma growth stimulating activity, alpha(MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.
Synonyms: Growth-regulated alpha protein,C-X-C motif chemokine 1,GRO-alpha(1-73),Melanoma growth stimulatory activity,MGSA,Neutrophil-activating protein 3,NAP-3,GRO-alpha(4-73),GRO-alpha(5-73),GRO-alpha(6-73),CXCL1,GRO, GRO1, GROA, MGSA, SCYB1,
Full Gene Name: Growth-regulated alpha protein
Cellular Localisation: Secreted. - 基因ID
- 2919
- UniProt
- P09341
- 途径
- Autophagy
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