我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

CXCL1 ELISA 试剂盒

Name: CXCL1 ELISA 试剂盒
SKU: ABIN411394
Availability: OutOfStock

This 大鼠 CXCL1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 CXCL1. This ELISA Kit has been cited in 2+ publications.

ELISA

1 image

(2 references)

产品编号 ABIN411394

发货至: 中国

Quick Overview for CXCL1 ELISA 试剂盒 (ABIN411394)

抗原

See all CXCL1 ELISA试剂盒

CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

抗原表位

AA 25-96

适用

大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6-1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (EDTA)

Discover our top product CXCL1 ELISA Kit

最低检测浓度

15.6 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CXCL1

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: A25-K96

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<1pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: A25-K96
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 适用: 猴 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 适用: 人 AA 35-107 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 适用: 小鼠 AA 20-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Serum

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Tissue Specificity: At least expressed in the lung and trachea.

板类型

Pre-coated

实验流程

rat CXCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL1 has been precoated onto 96-well plates. Standards(E. coli, A25-K96) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CXCL1 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat CXCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum or plasma(EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat CXCL1 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 75.4, Standard deviation: 4.1, CV(%): 5.4
- Sample 2: n=16, Mean(pg/ml): 221, Standard deviation: 13.7, CV(%): 6.2
- Sample 3: n=16, Mean(pg/ml): 546, Standard deviation: 28.4, CV(%): 5.2,
- Sample 1: n=24, Mean(pg/ml): 98.4, Standard deviation: 6, CV(%): 6.1
- Sample 2: n=24, Mean(pg/ml): 285, Standard deviation: 20.52, CV(%): 7.2
- Sample 3: n=24, Mean(pg/ml): 534, Standard deviation: 31.5, CV(%): 5.9
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Fu, Wang, Cai, Zhao, Fu: "Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa β-related pathway in rats." in: Drug design, development and therapy, Vol. 12, pp. 1609-1619, (2018) (PubMed).

Zhang, Wang, Liu, Zhang, Chen: "Suppressing Syndecan-1 Shedding Ameliorates Intestinal Epithelial Inflammation through Inhibiting NF-κB Pathway and TNF-α." in: Gastroenterology research and practice, Vol. 2016, pp. 6421351, (2016) (PubMed).
抗原 See all CXCL1 ELISA试剂盒

CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

别名

CXCL1

背景

Protein Function: Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation.
Background: Chemokine(C-X-C motif) ligand 1(CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3(NAP-3) and melanoma growth stimulating activity, alpha(MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.
Synonyms: Growth-regulated alpha protein,C-X-C motif chemokine 1,Cytokine-induced neutrophil chemoattractant 1,CINC-1,Platelet-derived growth factor-inducible protein KC,Cxcl1,Cinc1, Gro, Scyb1,
Full Gene Name: Growth-regulated alpha protein
Cellular Localisation: Secreted.

基因ID

81503

UniProt

P14095

途径

Autophagy