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NEDD4 抗体 (AA 173-262)

Name: NEDD4 抗体 (AA 173-262)
SKU: ABIN968557
Availability: OutOfStock

NEDD4 适用: 小鼠 WB, IF 宿主: 小鼠 Monoclonal 15-Nedd4 unconjugated

Western blot analysis of Nedd4 on a BC3H1 cell lysate (murine skeletal muscle cells). (NEDD4 抗体 (AA 173-262))

Immunoflourescence staining of RSV-3T3 cells. (NEDD4 抗体 (AA 173-262))

Image no. 3 for anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 173-262) antibody (ABIN968557) (NEDD4 抗体 (AA 173-262))

3 images

(4 references)

产品编号 ABIN968557

发货至: 中国

Datasheet as PDF

抗原 See all NEDD4 抗体

NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))
抗原表位
16

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 173-262
适用
38

26

13

5

4

2

2

2

1

1

1
小鼠
宿主
41

2
小鼠
Compatible Secondaries
克隆类型
39

3
单克隆
标记
26

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This NEDD4 antibody is un-conjugated
应用范围
41

19

13

13

13

12

8

5

3

1
Western Blotting (WB), Immunofluorescence (IF)

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Mouse Nedd4 aa. 173-262

克隆位点

15-Nedd4

亚型

IgG1

Top Product

Discover our top product NEDD4 Primary Antibody
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 1288-1319) antibody
NEDD4 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 160-460) antibody
NEDD4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 801-900) antibody
NEDD4 适用: 人, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) antibody
NEDD4 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 1288-1319), (C-Term) antibody
NEDD4 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB15462 unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) antibody
NEDD4 适用: 人, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 950-1000) antibody
NEDD4 适用: 人, 大鼠, 小鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 200-250) antibody
NEDD4 适用: 人, 大鼠, 小鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 324-373) antibody
NEDD4 适用: 人, 马, 犬, 兔, 猴, Cow, Bat WB 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (Internal Region) antibody
NEDD4 适用: 人, 马, Macaque WB, ELISA 宿主: 兔 Polyclonal unconjugated

说明

Related Products: ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
Peruzzi, Prisco, Morrione, Valentinis, Baserga: "Anti-apoptotic signaling of the insulin-like growth factor-I receptor through mitochondrial translocation of c-Raf and Nedd4." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 25990-6, (2001) (PubMed).

Harvey, Dinudom, Komwatana, Jolliffe, Day, Parasivam, Cook, Kumar: "All three WW domains of murine Nedd4 are involved in the regulation of epithelial sodium channels by intracellular Na+." in: The Journal of biological chemistry, Vol. 274, Issue 18, pp. 12525-30, (1999) (PubMed).

Plant, Yeger, Staub, Howard, Rotin: "The C2 domain of the ubiquitin protein ligase Nedd4 mediates Ca2+-dependent plasma membrane localization." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32329-36, (1998) (PubMed).

Hatakeyama, Jensen, Weissman: "Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases." in: The Journal of biological chemistry, Vol. 272, Issue 24, pp. 15085-92, (1997) (PubMed).
抗原

NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

别名

Nedd4 (NEDD4 产品)

别名

NEDD4-1 antibody, RPF1 antibody, AA959633 antibody, AL023035 antibody, AU019897 antibody, E430025J12Rik antibody, Nedd4-1 antibody, Nedd4a antibody, mKIAA0093 antibody, CG32184 antibody, CG42279 antibody, CG7555 antibody, DNedd4 antibody, Dmel\\CG42279 antibody, Dmel_CG32184 antibody, Dmel_CG7555 antibody, dNedd4 antibody, dNedd4-1 antibody, nedd4 antibody, GB16853 antibody, NEDD4 antibody, Nedd4 antibody, neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase antibody, neural precursor cell expressed, developmentally down-regulated 4 antibody, CG42279 gene product from transcript CG42279-RJ antibody, E3 ubiquitin-protein ligase Nedd-4 antibody, E3 ubiquitin-protein ligase NEDD4 antibody, NEDD4 antibody, Nedd4 antibody, LOC411723 antibody, LOC100457150 antibody, nedd4 antibody, LOC100547485 antibody

背景

Regulated protein degradation involves conjugation of ubiquitin to proteins for degradation by the 26S proteasome. Protein ubiquitination is the end product of a multienzyme cascade that involves sequential thiol-ester bond interactions between ubiquitin and enzymes termed E1-E3. Neuronal precursor cell-expressed developmentally down-regulated 4 (Nedd4) is a multimodular ubiquitin protein ligase E3 that contains a Ca 2+-lipid binding domain (CaLB), 3 WW domains, and a C-terminal ubiquitin protein ligase hect domain. The CaLB is characteristic of C2 domain-containing proteins, such as PKC, phospholipase C, phospholipase A2, and synaptogamin. Nedd4 is expressed differentially in the fetal and adult brain and its highest expression is in lung and kidney. Although it localizes in the cytoplasm, increased intracellular Ca 2+ induces plasma membrane association of Nedd4. In addition, increases in intracellular Na+ leads to Nedd4 binding to epithelial Na+ channels via its WW domains, which results in targeting of Na+ channels for degradation. Thus, the subcellular localization and protein interactions of Nedd4 are regulated in a Ca2+- and Na+-dependent manner. This antibody is routinely tested by western blot analysis.

分子量

110 kDa

途径

Notch Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2