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NEDD4 抗体 (Internal Region)

Name: NEDD4 抗体 (Internal Region)
Brand: antibodies-online
SKU: ABIN349606
Availability: OutOfStock

NEDD4 适用: 人, 马, Macaque WB, ELISA 宿主: 兔 Polyclonal unconjugated

Western blot using affinity purified anti-Nedd4 antibody shows detection of a 115 kDa band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates. (NEDD4 抗体 (Internal Region))

1 image

产品编号 ABIN349606

发货至: 中国

Datasheet as PDF

抗原 See all NEDD4 抗体

NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))
抗原表位
16

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
适用
37

26

13

4

4

2

2

2

1

1
人, 马, Macaque
宿主
40

2
兔
Compatible Secondaries
克隆类型
38

3
多克隆
标记
25

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This NEDD4 antibody is un-conjugated
应用范围
40

19

13

13

12

12

8

5

3

1
Western Blotting (WB), ELISA

特异性

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with endogenous Nedd4 protein. A BLAST analysis was used to suggest reactivity with Nedd4 from human, horse and macaque based on a 100% homology with the immunizing sequence. Expect reactivity with Nedd4 from chimpanzee, rabbit, dog, and cattle based on a 94% homology with the immunizing sequence. Cross-reactivity with Nedd4 from other sources has not been determined.

交叉反应

马, Macaque

产品特性

NEDD4 (neural precursor expressed, developmentally down-regulated protein 4, aliases: KIAA0093, MGC176705, NEDD4-1) is a gene that is highly expressed in early embryonic central nervous system. A family of NEDD4-like proteins has more recently been defined. NEDD4 and NEDD4-like proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4-2 have been shown to down-regulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations. The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins, and ubiquitination of these proteins, mediated by the HECT domain of NEDD4, results in their internalization and removal from the plasma membrane. Mutation of the PY motifs in ENaC proteins is associated with Liddle's Syndrome, an autosomal-dominant form of hypertension. In addition to targeting sodium channels, NEDD4-2 has also been shown to negatively regulate TGF-beta signaling by targeting Smad2 for degradation. Mouse and human NEDD4 are cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear.

纯化方法

affinity purified

过滤

Sterile filtered

免疫原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of the Nedd4 protein.
Immunogen Type: Peptide

亚型

IgG

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anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 1288-1319) antibody
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anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 160-460) antibody
NEDD4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 801-900) antibody
NEDD4 适用: 人, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) antibody
NEDD4 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 1288-1319), (C-Term) antibody
NEDD4 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB15462 unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) antibody
NEDD4 适用: 人, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 950-1000) antibody
NEDD4 适用: 人, 大鼠, 小鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 200-250) antibody
NEDD4 适用: 人, 大鼠, 小鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 324-373) antibody
NEDD4 适用: 人, 马, 兔, 犬, 猴, Cow, Bat WB 宿主: 兔 Polyclonal unconjugated

anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (Middle Region) antibody
NEDD4 适用: 人, 大鼠, 小鼠, 马, 兔, 犬, Cow, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated

应用备注

This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Nedd4 protein. Specific conditions for reactivity and detection of Nedd4 should be optimized by the end user. Expect a band approximately ~115 kDa in size corresponding to Nedd4 by western blotting in the appropriate cell lysate or extract.

说明

Gene Name: NEDD4

限制

仅限研究用
状态

Liquid

浓度

0.64 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

有效期

3 months
抗原

NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

别名

NEDD4 (NEDD4 产品)

别名

NEDD4-1 antibody, RPF1 antibody, AA959633 antibody, AL023035 antibody, AU019897 antibody, E430025J12Rik antibody, Nedd4-1 antibody, Nedd4a antibody, mKIAA0093 antibody, CG32184 antibody, CG42279 antibody, CG7555 antibody, DNedd4 antibody, Dmel\\CG42279 antibody, Dmel_CG32184 antibody, Dmel_CG7555 antibody, dNedd4 antibody, dNedd4-1 antibody, nedd4 antibody, GB16853 antibody, NEDD4 antibody, Nedd4 antibody, neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase antibody, neural precursor cell expressed, developmentally down-regulated 4 antibody, CG42279 gene product from transcript CG42279-RJ antibody, E3 ubiquitin-protein ligase Nedd-4 antibody, E3 ubiquitin-protein ligase NEDD4 antibody, NEDD4 antibody, Nedd4 antibody, LOC411723 antibody, LOC100457150 antibody, nedd4 antibody, LOC100547485 antibody

背景

NEDD4 (neural precursor expressed, developmentally down-regulated protein 4, aliases: KIAA0093, MGC176705, NEDD4-1) is a gene that is highly expressed in early embryonic central nervous system. A family of NEDD4-like proteins has more recently been defined. NEDD4 and NEDD4-like proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4-2 have been shown to down-regulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations. The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins, and ubiquitination of these proteins, mediated by the HECT domain of NEDD4, results in their internalization and removal from the plasma membrane. Mutation of the PY motifs in ENaC proteins is associated with Liddle's Syndrome, an autosomal-dominant form of hypertension. In addition to targeting sodium channels, NEDD4-2 has also been shown to negatively regulate TGF-β signaling by targeting Smad2 for degradation. Mouse and human NEDD4 are cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear.
Synonyms: E3 ubiquitin protein ligase Nedd4 antibody, KIAA0093 antibody, NEDD 4 antibody, Neural precursor cell expressed developmentally down regulated 4 antibody

基因ID

4734, 114520609

UniProt

P46934

途径

Notch Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2