(1 reference)-
- 抗原 See all VEGF 抗体
- VEGF (Vascular Endothelial Growth Factor (VEGF))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This VEGF antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human VEGF-165 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
- 产品特性
- VEGF (Vascular Endothelial Growth Factor A) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated. The sequence of this isoform differs from the canonical sequence as follows: 141-141: K -> N and 142-182: missing. This isoform is often found as a disulfide linked homodimer.
- 纯化方法
- purified
- 免疫原
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This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.
Immunogen Type: RecombinantProtein - 亚型
- IgG
- Top Product
- Discover our top product VEGF Primary Antibody
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anti-Vascular Endothelial Growth Factor (VEGF) (AA 27-120) antibody
VEGF 适用: 人, 小鼠, 大鼠, Pig, 山羊 WB, ELISA, IHC (p), FACS, IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠, 大鼠, Pig, 犬, Cow, 小鸡 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) (C-Term) antibodyVEGF 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB11705 unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 大鼠 WB, ELISA, IHC, FACS, IP, BR 宿主: 小鼠 Monoclonal A-4-6-1 unconjugated Recombinant Antibody
anti-Vascular Endothelial Growth Factor (VEGF) (AA 27-233) antibodyVEGF 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal 4E3 unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠 WB, IP 宿主: 小鼠 Monoclonal 6B7 unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) (full length) antibodyVEGF 适用: 人, 小鼠, Pig WB, IF 宿主: 小鸡 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibody (Biotin)VEGF 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal Biotin
anti-Vascular Endothelial Growth Factor (VEGF) antibody (Biotin)VEGF 适用: 人 WB, ELISA 宿主: 兔 Polyclonal Biotin
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- 应用备注
- This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 22 kDa in size corresponding to monomeric human VEGF-165 protein by western blotting in the appropriate cell lysate or extract.
- 说明
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Gene Name: VEGFA
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- Do NOT add Sodium Azide!
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
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: "The cannabinoid WIN 55,212-2 decreases specificity protein transcription factors and the oncogenic cap protein eIF4E in colon cancer cells." in: Molecular cancer therapeutics, Vol. 12, Issue 11, pp. 2483-93, (2014) (PubMed).
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: "The cannabinoid WIN 55,212-2 decreases specificity protein transcription factors and the oncogenic cap protein eIF4E in colon cancer cells." in: Molecular cancer therapeutics, Vol. 12, Issue 11, pp. 2483-93, (2014) (PubMed).
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- 抗原
- VEGF (Vascular Endothelial Growth Factor (VEGF))
- 别名
- VEGF (VEGF 产品)
- 别名
- MVCD1 antibody, VEGF antibody, VPF antibody, Vegf antibody, Vegf120 antibody, Vegf164 antibody, Vegf188 antibody, Vpf antibody, VEGF-A antibody, VEGF164 antibody, eVEGF120 antibody, eVEGF164 antibody, vegf-a antibody, vegf antibody, vascular endothelial growth factor A antibody, vascular endothelial growth factor A L homeolog antibody, vascular endothelial growth factor antibody, vascular endothelial growth factor precursor antibody, VEGFA antibody, Vegfa antibody, vegfa.L antibody, VEGF antibody, vegf antibody
- 背景
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VEGF (Vascular Endothelial Growth Factor A) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated. The sequence of this isoform differs from the canonical sequence as follows: 141-141: K → N and 142-182: missing. This isoform is often found as a disulfide linked homodimer.
Synonyms: Vascular endothelial growth factor A; VEGF-A; VEGF-165, VEGF165, VEGF isoform L, Vascular permeability factor; VPF - 基因ID
- 7422
- NCBI登录号
- NP_001165097
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