(3 references)-
- 抗原 See all VEGF 抗体
- VEGF (Vascular Endothelial Growth Factor (VEGF))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This VEGF antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- Biotin Conjugated VEGF Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human VEGF-165 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
- 产品特性
- VEGF (Vascular Endothelial Growth Factor A) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated. The sequence of this isoform differs from the canonical sequence as follows: 141-141: K -> N and 142-182: missing. This isoform is often found as a disulfide linked homodimer.
- 免疫原
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VEGF Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.
Immunogen Type: RecombinantProtein - 亚型
- IgG
- Top Product
- Discover our top product VEGF Primary Antibody
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anti-Vascular Endothelial Growth Factor (VEGF) (AA 27-120) antibody
VEGF 适用: 人, 小鼠, 大鼠, Pig, 山羊 WB, ELISA, IHC (p), FACS, IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠, 大鼠, Pig, 犬, Cow, 小鸡 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) (C-Term) antibodyVEGF 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB11705 unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 大鼠 WB, ELISA, IHC, FACS, IP, BR 宿主: 小鼠 Monoclonal A-4-6-1 unconjugated Recombinant Antibody
anti-Vascular Endothelial Growth Factor (VEGF) (AA 27-233) antibodyVEGF 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal 4E3 unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠 WB, IP 宿主: 小鼠 Monoclonal 6B7 unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) (full length) antibodyVEGF 适用: 人, 小鼠, Pig WB, IF 宿主: 小鸡 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibody (Biotin)VEGF 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal Biotin
anti-Vascular Endothelial Growth Factor (VEGF) antibody (HRP)VEGF 适用: 人 WB, ELISA 宿主: 兔 Polyclonal HRP
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- 应用备注
- VEGF conjugated antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 22 kDa in size corresponding to monomeric human VEGF-165 protein by western blotting in the appropriate cell lysate or extract.
- 说明
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Gene Name: VEGFA
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
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: "Deregulation of EGFR/VEGF/HIF-1a signaling pathway in colon adenocarcinoma based on tissue microarrays analysis." in: Journal of B.U.ON. : official journal of the Balkan Union of Oncology, Vol. 15, Issue 1, pp. 107-15, (2010) (PubMed).
: "Brain-derived neurotrophic factor induces proliferation, migration, and VEGF secretion in human multiple myeloma cells via activation of MEK-ERK and PI3K/AKT signaling." in: Tumour biology, Vol. 31, Issue 2, pp. 121-8, (2010) (PubMed).
: "New vascular endothelial growth factor isoform generated by internal ribosome entry site-driven CUG translation initiation." in: Molecular endocrinology (Baltimore, Md.), Vol. 15, Issue 12, pp. 2197-210, (2001) (PubMed).
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: "Deregulation of EGFR/VEGF/HIF-1a signaling pathway in colon adenocarcinoma based on tissue microarrays analysis." in: Journal of B.U.ON. : official journal of the Balkan Union of Oncology, Vol. 15, Issue 1, pp. 107-15, (2010) (PubMed).
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- 抗原
- VEGF (Vascular Endothelial Growth Factor (VEGF))
- 别名
- VEGF (VEGF 产品)
- 别名
- MVCD1 antibody, VEGF antibody, VPF antibody, Vegf antibody, Vegf120 antibody, Vegf164 antibody, Vegf188 antibody, Vpf antibody, VEGF-A antibody, VEGF164 antibody, eVEGF120 antibody, eVEGF164 antibody, vegf-a antibody, vegf antibody, vascular endothelial growth factor A antibody, vascular endothelial growth factor A L homeolog antibody, vascular endothelial growth factor antibody, vascular endothelial growth factor precursor antibody, VEGFA antibody, Vegfa antibody, vegfa.L antibody, VEGF antibody, vegf antibody
- 背景
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VEGF (Vascular Endothelial Growth Factor A) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated. The sequence of this isoform differs from the canonical sequence as follows: 141-141: K → N and 142-182: missing. This isoform is often found as a disulfide linked homodimer.
Synonyms: Vascular endothelial growth factor A; VEGF-A; VEGF-165, VEGF165, VEGF isoform L, Vascular permeability factor; VPF - 基因ID
- 7422
- NCBI登录号
- NP_001165097
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