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- 抗原 See all VEGF 抗体
- VEGF (Vascular Endothelial Growth Factor (VEGF))
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This VEGF antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine VEGF-A protein. A BLAST analysis was used to suggest cross-reactivity with VEGF-A from bovine, pig, sheep, and macaque sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against human, horse, dog, cat, or guinea pig based on 95% homology, and to rat and mouse based on 93% homology. Cross-reactivity with VEGF-A from other sources has not been determined.
- 产品特性
- VEGF is a potent mitogen in embryonic and somatic angiogenesis with specificity for vascular endothelial cells. VEGF forms homodimers and exists in four different isoforms. Overall, the VEGF monomer resembles that of PDGF, but its N-terminal segment is helical rather than extended. VEGF shares homologies of about 21% and 24% respectively with the A and B chains of human platelet-derived growth factor (PDGF), and has complete conservation of the eight cysteine residues found in both mature PDGF chains. The cysteine knot motif is a common feature of this domain. The homology is not reflected in function, however, since the cell types responsive to VEGF are distinct from those responsive to homo- and heterodimers of the PDGF chains. This protein is a glycosylated mitogen that acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. VEGF-A also has been shown to have effects on a number of other cell types (e.g. stimulation of monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells ). VEGF-A is also a vasodilator, it increases microvascular permeability, and was originally referred to as vascular permeability factor. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized.
- 纯化方法
- purified
- 过滤
- Sterile filtered
- 免疫原
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This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 164 amino acids of the mature bovine VEGF-A protein.
Immunogen Type: RecombinantProtein - 亚型
- IgG
- Top Product
- Discover our top product VEGF Primary Antibody
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anti-Vascular Endothelial Growth Factor (VEGF) (AA 27-120) antibody
VEGF 适用: 人, 小鼠, 大鼠, Pig, 山羊 WB, ELISA, IHC (p), FACS, IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠, 大鼠, Pig, 犬, Cow, 小鸡 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) (C-Term) antibodyVEGF 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB11705 unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 大鼠 WB, ELISA, IHC, FACS, IP, BR 宿主: 小鼠 Monoclonal A-4-6-1 unconjugated Recombinant Antibody
anti-Vascular Endothelial Growth Factor (VEGF) (AA 27-233) antibodyVEGF 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal 4E3 unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠 WB, IP 宿主: 小鼠 Monoclonal 6B7 unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) (full length) antibodyVEGF 适用: 人, 小鼠, Pig WB, IF 宿主: 小鸡 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibody (Biotin)VEGF 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal Biotin
anti-Vascular Endothelial Growth Factor (VEGF) antibody (Biotin)VEGF 适用: 人 WB, ELISA 宿主: 兔 Polyclonal Biotin
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- 应用备注
- This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.2 kDa in size corresponding to bovine VEGF-A protein by western blotting in the appropriate cell lysate or extract.
- 说明
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Gene Name: VEGFA
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
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- 抗原
- VEGF (Vascular Endothelial Growth Factor (VEGF))
- 别名
- VEGF (VEGF 产品)
- 别名
- MVCD1 antibody, VEGF antibody, VPF antibody, Vegf antibody, Vegf120 antibody, Vegf164 antibody, Vegf188 antibody, Vpf antibody, VEGF-A antibody, VEGF164 antibody, eVEGF120 antibody, eVEGF164 antibody, vegf-a antibody, vegf antibody, vascular endothelial growth factor A antibody, vascular endothelial growth factor A L homeolog antibody, vascular endothelial growth factor antibody, vascular endothelial growth factor precursor antibody, VEGFA antibody, Vegfa antibody, vegfa.L antibody, VEGF antibody, vegf antibody
- 背景
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VEGF is a potent mitogen in embryonic and somatic angiogenesis with specificity for vascular endothelial cells. VEGF forms homodimers and exists in four different isoforms. Overall, the VEGF monomer resembles that of PDGF, but its N-terminal segment is helical rather than extended. VEGF shares homologies of about 21% and 24% respectively with the A and B chains of human platelet-derived growth factor (PDGF), and has complete conservation of the eight cysteine residues found in both mature PDGF chains. The cysteine knot motif is a common feature of this domain. The homology is not reflected in function, however, since the cell types responsive to VEGF are distinct from those responsive to homo- and heterodimers of the PDGF chains. This protein is a glycosylated mitogen that acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. VEGF-A also has been shown to have effects on a number of other cell types (e.g. stimulation of monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells ). VEGF-A is also a vasodilator; it increases microvascular permeability, and was originally referred to as vascular permeability factor. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized.
Synonyms: Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF - 基因ID
- 281572, 27806357
- UniProt
- P15691
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