Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
纯化方法
CCDC98 Antibody is affinity chromatography purified via peptide column.
免疫原
CCDC98 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human CCDC98. The immunogen is located within amino acids 80 - 130 of CCDC98.
FAM175A
适用: 小鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 350
应用备注
CCDC98 antibody can be used for detection of CCDC98 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
CCDC98 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
CCDC98 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
CCDC98 (FAM175A)
(Family with Sequence Similarity 175, Member A (FAM175A))
CCDC98 antibody, abra1 antibody, ccdc98 antibody, fam175a antibody, ABRA1 antibody, 3830405G04Rik antibody, 5630400M01Rik antibody, AI506069 antibody, AL024423 antibody, AV118690 antibody, Ccdc98 antibody, RGD1305287 antibody, zgc:103522 antibody, abraxas 1, BRCA1 A complex subunit antibody, Coiled-coil domain-containing protein 98 antibody, BRCA1 A complex subunit antibody, family with sequence similarity 175 member A antibody, abraxas 2a, BRISC complex subunit antibody, abraxas 1, BRCA1 A complex subunit S homeolog antibody, ABRAXAS1 antibody, abraxas1 antibody, Abraxas1 antibody, FAM175A antibody, abraxas2a antibody, abraxas1.S antibody
背景
CCDC98 Antibody: CCDC98, also known as Abraxas 1, was identified through protein binding studies using the breast and ovarian predisposition protein BRCA1 as the binding target. CCDC98 recruits RAP80, a ubiquitin-binding protein, to BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both CCDC98 and RAP80 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either CCDC98 or RAP80 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that CCDC98 and RAP80 control only part of the DNA damage response role of BRCA1. At least two isoforms of CCDC98 are known to exist.