Cytochrome C 抗体 (AA 1-80)
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- 抗原 See all Cytochrome C (CYCS) 抗体
- Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
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抗原表位
- AA 1-80
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适用
- 人, 大鼠, 小鼠, 马, 犬, Pigeon, Drosophila melanogaster, Frog
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Cytochrome C antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
- 特异性
- It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
- 克隆位点
- SPM389
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product CYCS Primary Antibody
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anti-Cytochrome C, Somatic (CYCS) (AA 51-105) antibody
CYCS 适用: 人, 大鼠, 小鼠, 兔, 小鸡 WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 1-105) antibodyCYCS 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 5-100) antibodyCYCS 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 13-99) antibodyCYCS 适用: 大鼠, 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 5-100) antibodyCYCS 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 1268-1341) antibodyCYCS 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 1-105) antibodyCYCS 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5E6B11 unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 2-105) antibodyCYCS 适用: 人, 大鼠, 小鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 2-105) antibodyCYCS 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C, Somatic (CYCS) (AA 91-105), (C-Term) antibodyCYCS 适用: 人, 大鼠, 小鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
- 别名
- CYCS (CYCS 产品)
- 别名
- CYC antibody, HCS antibody, THC4 antibody, cyct antibody, cyc antibody, hcs antibody, CYCS antibody, cytc antibody, CYCSA antibody, CG17903 antibody, Cyt C antibody, Cyt c antibody, Cyt-c2 antibody, CytC-2 antibody, Cytc antibody, Cytc-p antibody, DC4 antibody, DMc01 antibody, Dmel\\CG17903 antibody, cyt c antibody, cyt c-p antibody, cyt-c-p antibody, cyt-c2 antibody, cyt.c antibody, cytochrome c antibody, dc4 antibody, pDMc01 antibody, wu:fk52b01 antibody, zgc:86706 antibody, ENSMUSG00000058927 antibody, cytochrome c, somatic antibody, cytochrome c, somatic S homeolog antibody, Cytochrome c proximal antibody, cytochrome c, somatic b antibody, cytochrome c, somatic pseudogene antibody, cytochrome c-like antibody, cytochrome c antibody, CYCS antibody, cycs.S antibody, cycs antibody, Cycs antibody, Cyt-c-p antibody, cycsb antibody, Gm10053 antibody, LOC100724240 antibody, CYC antibody, LOC100847700 antibody, LOC101107954 antibody, LOC106829744 antibody, LOC106992390 antibody, LOC102085712 antibody
- 分子量
- 15kDa
- 基因ID
- 54205
- UniProt
- P99999
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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