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NR1I2 抗体 (AA 108-157)

NR1I2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN6741564
发货至: 中国
  • 抗原 See all NR1I2 抗体
    NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))
    抗原表位
    • 16
    • 15
    • 7
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    • 4
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    • 3
    • 2
    • 1
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    AA 108-157
    适用
    • 69
    • 43
    • 28
    • 18
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    宿主
    • 75
    • 8
    • 1
    克隆类型
    • 78
    • 6
    多克隆
    标记
    • 38
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
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    • 1
    This NR1I2 antibody is un-conjugated
    应用范围
    • 78
    • 28
    • 19
    • 18
    • 13
    • 13
    • 12
    • 7
    • 7
    • 4
    • 4
    • 3
    • 2
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human NR1I2 / PXR
    预测反应
    Percent identity by BLAST analysis: Human (100%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic peptide located between aa108-157 of human NR1I2 (O75469, NP_003880). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey (85%).

    Type of Immunogen: Synthetic peptide
    亚型
    IgG
    Top Product
    Discover our top product NR1I2 Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Distilled water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS with 2 % sucrose
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原
    NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))
    别名
    NR1I2 / PXR / PAR (NR1I2 产品)
    别名
    BXR antibody, ONR1 antibody, PAR antibody, PAR1 antibody, PAR2 antibody, PARq antibody, PRR antibody, PXR antibody, SAR antibody, SXR antibody, PXR.1 antibody, PXR.2 antibody, PXR1 antibody, mPXR antibody, bxr antibody, nr1i2 antibody, onr1 antibody, xonr1 antibody, nuclear receptor subfamily 1 group I member 2 antibody, nuclear receptor subfamily 1, group I, member 2 antibody, nuclear receptor subfamily 1 group I member 2 S homeolog antibody, NR1I2 antibody, Nr1i2 antibody, nr1i2.S antibody, nr1i2 antibody
    背景
    Name/Gene ID: NR1I2
    Subfamily: NR1 Thyroid hormone-like
    Family: NHR

    Synonyms: NR1I2, BXR, Orphan nuclear receptor PXR, Orphan nuclear receptor PAR1, PARq, PRR, PXR, Pregnane X receptor, ONR1, SAR, SXR
    基因ID
    8856
    NCBI登录号
    NP_003880
    UniProt
    O75469
    途径
    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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