Cytokeratin 8/18 抗体 (Biotin)
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- 抗原 See all Cytokeratin 8/18 products
- Cytokeratin 8/18
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Cytokeratin 8/18 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-Cytokeratin 8/18 (KRT8/803 + KRT18/835), Biotin Conjugate
- 产品特性
- Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immuno-histochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Recombinant full-length human KRT8 protein (KRT8/803), Recombinant full-length human KRT18 protein (KRT18/835)
- 克隆位点
- KRT8-803 KRT18-835
- 亚型
- IgG
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anti-Cytokeratin 8/18 antibody
适用: 人 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal B22-1-B23-1 unconjugated
anti-Cytokeratin 8/18 antibody适用: 人 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal KRT8-803-KRT18-835 unconjugated
anti-Cytokeratin 8/18 antibody适用: 人 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal KRT8-803-KRT18-835 unconjugated
anti-Cytokeratin 8/18 antibody适用: 人, Cow, 猴, Pig, 绵羊 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal C-51 unconjugated
anti-Cytokeratin 8/18 antibody适用: 人, Cow, 猴, Pig, 绵羊 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal C-51 unconjugated
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- 应用备注
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Immunohistology formalin-fixed 0.5-1.0 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1.0 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
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MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- 抗原
- Cytokeratin 8/18
- Abstract
- Cytokeratin 8/18 产品
- 背景
- P145, Steel Factor Receptor, Stem Cell Factor Receptor (SCF-Receptor), Mast Cell Growth Factor Receptor
- 分子量
- 52.5 kDa (CK8), 45 kDa (CK18)
- 基因ID
- 3856, 3875
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