Cytokeratin 8/18 抗体
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北京 101111
Quick Overview for Cytokeratin 8/18 抗体 (ABIN3024678)
抗原
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- Cytokeratin 8/18 Antibody (azide and preservative free)
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无交叉反应
- 小鼠, 大鼠, 雪貂, 兔, 驴, 小鸡, 非洲爪蟾
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纯化方法
- Protein G affinity chromatography
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免疫原
- A cytoskeleton preparation from HeLa cells was used as the immunogen for the Cytokeratin 8/18 antibody.
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亚型
- IgG1, kappa
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anti-Cytokeratin 8/18 antibody
适用: 人 IF, WB, FACS, IHC 宿主: 小鼠 Monoclonal B22-1-B23-1 unconjugated
anti-Cytokeratin 8/18 antibody适用: 人 IHC (p) 宿主: 小鼠 Monoclonal KRT8-803-KRT18-835 unconjugated
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应用备注
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Optimal dilution of the Cytokeratin 8/18 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Cytokeratin 8/18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Cytokeratin 8/18
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背景
- Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
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UniProt
- P05787
抗原
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