IL1A 抗体 (Cleavage Site)
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- 抗原 See all IL1A 抗体
- IL1A (Interleukin 1 alpha (IL1A))
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抗原表位
- Cleavage Site
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IL1A antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 产品特性
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Synonyms: Interleukin 1 alpha precursor antibody, rabbit anti-Interleukin1 alpha antibody, rabbit anti-IL-1 Alpha Antibody, Pre-interleukin 1 alpha antibody
Background: Interleukin 1 alpha is produced by activated macrophages. Anti-IL-1 alpha antibody is ideal for investigators involved in Immunology research.
Gene Name: IL1A
- 纯化方法
- This is an IgG preparation of whole rabbit serum purified by DEAE fractionation. This antibody is primarily directed against the 17,000 MW human IL-1a and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1a in the same formats using similar dilutions. The antiserum does not recognize human IL-1b or Mouse or Rabbit IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize both the mature 17,000 MW IL-1a as well as the 31,000 MW IL-1a precursor in either non-denatured (native) or denatured samples. Unlike the IL-1b precursor, the native precursor of IL-1a is recognized by the antibody produced to the 17,000 MW form. The 31,000 precursor of IL-1a is biologically active and is found primarily intracellularly. The precursor of IL-1a, unlike that of IL-1b, is biologically active when applied to cells and is thought to have a role as a functional molecule intracellularly and can be found constitutively expressed in various cell. This antibody is also useful for neutralization of human and primate IL-1a activity in bioassays. It does not neutralize the biological activity IL-1b. It does not neutralize the biological activity of mouse, rat or rabbit IL-1a. It will neutralize primate IL-a. For neutralization, it is recommended to incubate the sample with a 1:100 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.
- 免疫原
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Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli. The MW of the recombinant IL-1a was 17,000. This is the cleavage site generated by the IL-1b converting enzyme (ICE, capase-1).
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product IL1A Primary Antibody
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anti-Interleukin 1 alpha (IL1A) (AA 172-271) antibody
IL1A 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 4F9 unconjugated
anti-Interleukin 1 alpha (IL1A) antibodyIL1A 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 6G4A5 unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 177-206) antibodyIL1A 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21521 unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 113-271) antibodyIL1A 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C13 unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 90-268) antibodyIL1A 适用: 山羊 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 112-271) antibodyIL1A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 177-206) antibodyIL1A 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 115-270) antibodyIL1A 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Interleukin 1 alpha (IL1A) (N-Term) antibodyIL1A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 alpha (IL1A) (AA 151-220) antibodyIL1A 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:100 to 1:200
Application Note: This IgG fraction antibody of anti-Human IL-1a has been tested for use in neutralizations, ELISA, immunohistochemistry, flow cytometry and immunoblotting. It recognizes the 17,000 MW mature IL-1a. Reactivity in other immunoassays is unknown.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IL1A (Interleukin 1 alpha (IL1A))
- 别名
- IL-1 Alpha (IL1A 产品)
- 别名
- IL-1A antibody, IL1 antibody, IL1-ALPHA antibody, IL1F1 antibody, IL-6 antibody, Il-1a antibody, IL-1 alpha antibody, IL-1alpha antibody, L1A antibody, interleukin 1 alpha antibody, interleukin 6 antibody, IL1A antibody, IL6 antibody, Il1a antibody
- 基因ID
- 3552
- UniProt
- P01583
- 途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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