Transcription factor E2F1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Transcription factor E2F1 Antibody was raised against synthetic peptide QEVTSEEENRATDS found at an internal region of Transcription factor E2F1.
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 60 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 46.9 kDa according to NP_005216.1). Recommended concentration: 1-3μ,g/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Transcription factor E2F1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot and store at -20°C. Minimize freezing and thawing.