Collagen IV 抗体
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- 抗原 See all Collagen IV (COL4) 抗体
- Collagen IV (COL4) (Collagen, Type IV (COL4))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Collagen IV antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)
- 特异性
- Human collagen type IV 100 % human collagen types I, II, III, and V <0.1 % human fibronectin and laminin < 0.1 % No cross-reactivity with bovine
- 无交叉反应
- Cow, 小鼠, Pig, 大鼠
- 交叉反应 （详细）
- Human, no cross-reactivity with bovine, mouse, rat and porcine collagens type IV
- 产品特性
- Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type IV 100 % , human collagen types I, II, III, and V <0.1 %, human fibronectin and laminin < 0.1 % (RIA at 1:10000 dilution).
- 纯化方法
- Protein G affinity purified antibody prod. in nude mice.
- 免疫原
- Purified collagen type IV from human placenta
- 克隆位点
- 24D1
- 亚型
- IgG1
- Top Product
- Discover our top product COL4 Primary Antibody
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anti-Collagen, Type IV (COL4) antibody
COL4 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1571-1669) antibodyCOL4 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1401-1500) antibodyCOL4 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA, IHC, IHC (fro), IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-704R unconjugated Recombinant Antibody
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人, 大鼠, Pig, Cow WB, IHC, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal LP46 unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 WB, IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal 1043 unconjugated
anti-Collagen, Type IV (COL4) antibody (FITC)COL4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F11 FITC
anti-Collagen, Type IV (COL4) antibody (Biotin)COL4 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal Biotin
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- 应用备注
- After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Human skin, placenta or lens capsule,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:320, IHC(P) ? 1:4000, ELISA ? 1:4000 (OD ? 500)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- Lot specific
- 缓冲液
- phosphate buffered solution, no BSA and preservative added!
- 储存液
- Without preservative
- 储存条件
- -20 °C
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- 抗原
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- 别名
- Collagen Type IV (COL4 产品)
- 别名
- collagen, type IV antibody, Let-2 antibody
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