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Collagen IV 抗体

COL4 适用: 人, 大鼠, Pig, Cow WB, IHC, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal LP46 unconjugated
产品编号 ABIN335405
发货至: 中国
  • 抗原 See all Collagen IV (COL4) 抗体
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    适用
    • 90
    • 43
    • 40
    • 22
    • 16
    • 10
    • 5
    • 2
    人, 大鼠, Pig, Cow
    宿主
    • 60
    • 26
    • 13
    小鼠
    克隆类型
    • 72
    • 27
    单克隆
    标记
    • 49
    • 10
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Collagen IV antibody is un-conjugated
    应用范围
    • 61
    • 50
    • 30
    • 29
    • 26
    • 26
    • 26
    • 26
    • 20
    • 9
    • 9
    • 8
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    特异性
    Human, rat, pig, cow.
    纯化方法
    Purified
    免疫原
    1043 is a mouse monoclonal IgG2b antibody derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with purified human type IV collagen. The immunogen has been isolated from human placenta.
    克隆位点
    LP46
    亚型
    IgG1
    Top Product
    Discover our top product COL4 Primary Antibody
  • 应用备注
    1043 recognizes the extracellular - basement membranes. 1043 is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl 2 . For immunohistochemistry on paraffin sections the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. Optimal antibody dilution should be determined by titration, 1:50 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
    限制
    仅限研究用
  • 储存条件
    4 °C
  • Schapers, Pauwels, Havenith, Smeets, van den Brandt, Bosman: "Prognostic significance of type IV collagen and laminin immunoreactivity in urothelial carcinomas of the bladder." in: Cancer, Vol. 66, Issue 12, pp. 2583-8, (1991) (PubMed).

    Cleutjens, Havenith, Kasper, Vallinga, Bosman: "Absence of type IV collagen in the centre of the corneal epithelial basement membrane." in: The Histochemical journal, Vol. 22, Issue 12, pp. 688-94, (1991) (PubMed).

    Cleutjens, Havenith, Beek, Vallinga, Ten Kate, Bosman: "Origin of basement membrane type IV collagen in xenografted human epithelial tumor cell lines." in: The American journal of pathology, Vol. 136, Issue 5, pp. 1165-72, (1990) (PubMed).

    Visser, van der Beek, Havenith, Cleutjens, Bosman: "Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia." in: Histopathology, Vol. 10, Issue 2, pp. 171-80, (1986) (PubMed).

  • 抗原
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    别名
    Collagen Type IV (COL4 产品)
    别名
    collagen, type IV antibody, Let-2 antibody
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