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GFP 抗体 (GFP tag)

Name: GFP 抗体 (GFP tag)
Brand: antibodies-online
SKU: ABIN5596892
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, ELISA, IHC, FACS, FM 宿主: 小鼠 Monoclonal 9F9-F9 GFP tag

Western Blot of monoclonal anti-GFP antibody. (GFP 抗体 (GFP tag))

Mouse monoclonal Anti-GFP antibody is able to detect positive cell population in cytoflow analysis. (GFP 抗体 (GFP tag))

FACS

SDS PAGE of Mouse anti-GFP antibody. (GFP 抗体 (GFP tag))

SDS

Western Blot of Mouse anti-GFP antibody. (GFP 抗体 (GFP tag))

4 images

产品编号 ABIN5596892

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
174

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
宿主
74

72

35

11

9

6

2

1
小鼠
Compatible Secondaries
克隆类型
121

84
单克隆
标记
104

14

12

9

6

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to GFP tag
应用范围
172

86

55

41

33

31

28

20

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

交叉反应（详细）

Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215) from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP.

纯化方法

GFP Monoclonal Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum.

免疫原

Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
Immunogen Type: Recombinant Protein

克隆位点

9F9-F9

亚型

IgG

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应用备注

Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
Western Blot Dilution: 1:3000 - 1:30,000
ELISA Dilution: 1:80,000 - 1:500,000
IF Microscopy Dilution: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store mouse anti-GFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

物质类

Viral Protein

背景

Synonyms: Green Fluorescent protein, GFP
Background: Mouse anti-GFP antibody is functional by western blot, ELISA, Immunofluorescene Micorscopy and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.