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GFP 抗体

(Green Fluorescent Protein (GFP))

Green fluorescent protein (GFP) antibodies play a crucial role in various research areas, including cell biology, molecular biology, developmental biology, immunology, and neuroscience, providing valuable tools for visualizing and studying protein localization, expression, interactions, and dynamics. GFP is a naturally occurring fluorescent protein derived from the jellyfish Aequorea victoria, and it emits green light when exposed to blue or ultraviolet light. Antibodies specific to GFP can recognize and bind to the GFP protein, enabling researchers to visualize and study GFP-tagged proteins or cells in various experimental settings. Common use cases for GFP antibodies in research include:

  • GFP antibodies are widely used to detect fusion proteins via Western blotting. A fusion protein is created by genetically fusing the GFP gene to a gene of interest, resulting in the production of a chimeric protein that contains both the GFP portion and the protein of interest. The GFP moiety acts as a tag, allowing researchers to track and visualize the fusion protein within cells or tissues. The resulting signal on the Western blot indicates the presence and size of the fusion protein. Secondary antibodies conjugated to an enzyme, such as horseradish peroxidase (HRP) can be used to amplify the signal.
    This approach allows researchers to study the expression and detection of fusion proteins. Blotting is commonly used in molecular biology and cell biology research to confirm the successful production of fusion proteins, assess their expression levels, and investigate their interactions and localization within cells.
    Suitable for WB and ELISA experiments: anti-GFP Antibody
  • Protein expression analysis: GFP antibodies can be employed to analyze protein expression levels in cells or tissues. Researchers can use immunoblotting techniques, such as Western blotting, to detect and quantify GFP-tagged proteins by specifically binding to the GFP portion. This helps in assessing protein expression levels and monitoring changes under different experimental conditions.
  • Protein localization: GFP fusion proteins are commonly used to track the localization of proteins within cells. By introducing a GFP-tagged protein into cells and using GFP antibodies, researchers can visualize and determine the subcellular localization of the protein of interest. This allows for the study of protein dynamics and cellular processes.
  • Flow cytometry and cell sorting: Flow cytometry allows for the analysis and sorting of cells based on their fluorescent properties. GFP antibodies are employed to detect and quantify GFP-expressing cells in heterogeneous populations. This enables researchers to isolate and characterize specific cell populations based on GFP expression, facilitating the study of cell types, differentiation, or disease states.
  • Immunofluorescence and microscopy: GFP antibodies are utilized in immunofluorescence experiments to visualize and localize GFP-tagged proteins within fixed cells or tissues. By using secondary antibodies conjugated to fluorophores, such as Alexa Fluor or DyLight dyes, the GFP antibodies can be detected and visualized under a fluorescence microscope. This technique enables researchers to study protein localization, interactions, and dynamics in real-time.
    Suitable for FACS and IF experiments: Recombinant anti-GFP Single-Domain Antibody
  • Co-localization studies: GFP antibodies are essential tools for studying protein-protein interactions and co-localization within cells. By using multiple antibodies, including GFP antibodies, against different target proteins, researchers can perform co-immunoprecipitation or immunofluorescence experiments to investigate the spatial relationships and potential interactions between the GFP-tagged protein and other proteins of interest.

antibodies-online offers a wide range of GFP antibodies. Have a look at our top GFP antibodies or discover GFP antibodies tailored to your experimental needs down below!

GFP 抗体 GFP 抗体 GFP 抗体 (ABIN100085)

GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated

GFP 抗体 GFP 抗体 GFP 抗体 (ABIN1439999)

GFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated

GFP 抗体 GFP 抗体 GFP 抗体 (ABIN7141875)

GFP WB, ELISA, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated

GFP 抗体 by 适用

Find GFP 抗体 for a variety of species such as anti-Aequorea victoria GFP, anti-Tag GFP, anti-Aequorea coerulescens (Belt Jellyfish) GFP. The species listed below are among those available. Click on a link to go to the corresponding products.

GFP 抗体 by 应用范围

Find GFP 抗体 validated for a specific application such as WB, ELISA, IF, IHC. Some of the available applications are listed below. Click on a link to go to the corresponding products.

GFP 抗体 by 抗体来源

Find GFP 抗体 with a specific 抗体来源. The 抗体来源 listed below are among those available. Click on a link to go to the corresponding products.

GFP 抗体 by 抗原表位

Find GFP 抗体 with a specific epitope. The epitopes listed below are among those available. Click on a link to go to the corresponding products.

GFP 抗体 by 克隆形成能力

Find available monoclonal or polyclonal GFP 抗体. Click on a link to go to the corresponding products.

GFP 抗体 by 克隆

Find GFP 抗体 with a specific 克隆. The 克隆 listed below are among those available. Click on a link to go to the corresponding products.

GFP 抗体 by 标记

Find GFP 抗体 with a specific conjugate such as Biotin, FITC, HRP. The conjugates listed below are among those available. Click on a link to go to the corresponding products.

GFP 抗体 by Fragment

Find GFP 抗体 with a specific Fragment. The Fragment listed below are among those available. Click on a link to go to the corresponding products.

Popular GFP 抗体

Product
Reactivity
Application
Validations
Cat. No.
Quantity
Datasheet
Reactivity Aequorea victoria
Application WB, ELISA, FM
Validations
  • (178)
  • (15)
Cat. No. ABIN100085
Quantity 100 μL
Datasheet Datasheet
Reactivity Aequorea aequorea
Application WB, IF, IHC (fro), IHC (p)
Validations
  • (18)
  • (5)
Cat. No. ABIN1439999
Quantity 1 mg
Datasheet Datasheet
Reactivity
Application WB, ELISA, IF, IP, FACS
Validations
  • (7)
Cat. No. ABIN7141875
Quantity 100 μg
Datasheet Datasheet
Reactivity Aequorea victoria
Application WB, ELISA
Validations
  • (17)
  • (4)
Cat. No. ABIN129564
Quantity 1 mg
Datasheet Datasheet
Reactivity Aequorea victoria
Application WB, ELISA, IP, IC, ChIP-seq
Validations
  • (1)
  • (7)
Cat. No. ABIN2451988
Quantity 100 μg
Datasheet Datasheet
Reactivity Aequorea victoria
Application WB, ELISA, IP
Validations
  • (13)
  • (3)
Cat. No. ABIN1574093
Quantity 40 μg
Datasheet Datasheet
Reactivity Aequorea victoria
Application WB, IF, IHC (fro), IHC (p), IEM
Validations
  • (5)
Cat. No. ABIN6254149
Quantity 300 μg
Datasheet Datasheet
Reactivity Aequorea victoria
Application WB, ELISA, FM
Validations
  • (43)
  • (2)
Cat. No. ABIN129570
Quantity 100 μg
Datasheet Datasheet
Reactivity Aequorea victoria
Application WB, ELISA, IHC, FACS
Validations
  • (3)
  • (4)
Cat. No. ABIN1724673
Quantity 100 μL
Datasheet Datasheet
Reactivity Aequorea victoria
Application FACS, IM
Validations
  • (8)
  • (4)
Cat. No. ABIN100088
Quantity 1 mg
Datasheet Datasheet
Reactivity Aequorea victoria
Application WB, ELISA, FM
Validations
  • (9)
  • (3)
Cat. No. ABIN349609
Quantity 100 μg
Datasheet Datasheet
Reactivity
Application WB, IF
Validations
  • (3)
  • (3)
Cat. No. ABIN3020573
Quantity 100 μL
Datasheet Datasheet
Reactivity
Application WB, IF
Validations
  • (5)
  • (3)
Cat. No. ABIN3020571
Quantity 100 μL
Datasheet Datasheet
Reactivity Aequorea victoria
Application WB, ELISA, IHC, FACS, FM
Validations
  • (4)
Cat. No. ABIN5596892
Quantity 100 μg
Datasheet Datasheet
Reactivity Aequorea victoria
Application WB, IF, IHC (fro)
Validations
  • (4)
Cat. No. ABIN7273061
Quantity 250 μg
Datasheet Datasheet

Latest Publications for our GFP 抗体

Lapraz, Boutres, Fixary-Schuster, De Queiroz, Plaçais, Cerezo, Besse, Préat, Noselli: "Asymmetric activity of NetrinB controls laterality of the Drosophila brain." in: Nature communications, Vol. 14, Issue 1, pp. 1052, (2023) (PubMed).

Twittenhoff, Heroven, Mühlen, Dersch, Narberhaus: "An RNA thermometer dictates production of a secreted bacterial toxin." in: PLoS pathogens, Vol. 16, Issue 1, pp. e1008184, (2020) (PubMed).

Nihei, Mori, Werner, Arzberger, Zhou, Khosravi, Japtok, Hermann, Sommacal, Weber, Kamp, Nuscher, Edbauer, Haass: "Poly-glycine-alanine exacerbates C9orf72 repeat expansion-mediated DNA damage via sequestration of phosphorylated ATM and loss of nuclear hnRNPA3." in: Acta neuropathologica, Vol. 139, Issue 1, pp. 99-118, (2020) (PubMed).

Madsen, Jones, Tucker, Giler, Muller, Discher, Russell, Dobek, Sammarco, Bunnell, OConnor: "Survival of aging CD264+ and CD264- populations of human bone marrow mesenchymal stem cells is independent of colony-forming efficiency." in: Biotechnology and bioengineering, Vol. 117, Issue 1, pp. 223-237, (2020) (PubMed).

Zhang, Tung, Wang, Yu, Gross, Wei, Berglund: "Improved trafficking and expression of luminopsins for more efficient optical and pharmacological control of neuronal activity." in: Journal of neuroscience research, Vol. 98, Issue 3, pp. 481-490, (2020) (PubMed).

Shen, Kilander, Bridi, Frei, Niescier, Huang, Lin: "Tomosyn regulates the small RhoA GTPase to control the dendritic stability of neurons and the surface expression of AMPA receptors." in: Journal of neuroscience research, Vol. 98, Issue 6, pp. 1213-1231, (2020) (PubMed).

Choi, Kim, Song, Cha, Cho, Son, Kim, Mook-Jung: "Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death." in: Aging cell, Vol. 18, Issue 1, pp. e12872, (2020) (PubMed).

Cone, Hurwitz, Lee, Yuan, Zhou, Li, Meckes: "Alix and Syntenin-1 direct amyloid precursor protein trafficking into extracellular vesicles." in: BMC molecular and cell biology, Vol. 21, Issue 1, pp. 58, (2020) (PubMed).

Kim, Kim, Choi, Lee, Lee, Im, Shin, Kim, Hong, Kim, Kim, Sung: "Downregulated miR-18b-5p triggers apoptosis by inhibition of calcium signaling and neuronal cell differentiation in transgenic SOD1 (G93A) mice and SOD1 (G17S and G86S) ALS patients." in: Translational neurodegeneration, Vol. 9, Issue 1, pp. 23, (2020) (PubMed).

Saegusa, Hosoya, Nishiyama, Saeki, Fujimoto, Okano, Fujioka, Ogawa: "Low-dose rapamycin-induced autophagy in cochlear outer sulcus cells." in: Laryngoscope investigative otolaryngology, Vol. 5, Issue 3, pp. 520-528, (2020) (PubMed).

Aliases for GFP 抗体

green fluorescent protein (gfp) 抗体
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