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GFP 抗体

Name: GFP 抗体
Brand: antibodies-online
SKU: ABIN302066
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, IP, ICC 宿主: 兔 Polyclonal unconjugated

Confocal microscopy Confocal microscopy images of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP Fig.

Immunocytochemistry staining (confocal microscopy) of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP, top) or nuclear Polycomb 2-EYFP fusion protein (Pc2-EYFP, bottom).

ICC

Confocal microscopy Confocal microscopy images of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP; top) or nuclear Polycomb 2-EYFP fusion protein (Pc2-EYFP; bottom).

3 images

(2 references)

产品编号 ABIN302066

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
174

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
宿主
73

73

35

11

9

6

2

1
兔
Compatible Secondaries
克隆类型
120

85
多克隆
标记
103

14

12

9

6

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
172

87

55

42

32

30

29

21

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

特异性

The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species.

交叉反应（详细）

Recognizes fusion proteins in all species

纯化方法

Purified from rabbit serum by affinity chromatography

纯度

> 95 % (by SDS-PAGE)

免疫原

EGFP, a native full-length protein

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anti-Green Fluorescent Protein (GFP) antibody
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GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 兔 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
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anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)
GFP 适用: Aequorea victoria FACS, IM 宿主: 山羊 Polyclonal FITC

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Porrero, Rubio-Garrido, Avendauno, Clascua: "Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice." in: Brain research, (2010) (PubMed).

Valenta, Lukas, Doubravska, Fafilek, Korinek: "HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies." in: The EMBO journal, Vol. 25, Issue 11, pp. 2326-37, (2006) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

物质类

Viral Protein

背景

Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (λ,=509 nm) when excited (excitation by Blue or UV light, absorption peak λ,=395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.