GFP 抗体 (AA 246)
(43 references)-
- 抗原 See all GFP 抗体
- GFP (Green Fluorescent Protein (GFP))
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抗原表位
- AA 246
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适用
- Aequorea victoria
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GFP antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
- 交叉反应
- recombinant GFP (rGFP), enhanced GFP (eGFP), S65T-GFP, RS-GFP, YFP
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 免疫原
- The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.br Immunogentype:Recombinant
- 亚型
- IgG
- Top Product
- Discover our top product GFP Primary Antibody
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anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP WB, ELISA, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibodyGFP 适用: Aequorea victoria WB, ELISA 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)GFP 适用: Aequorea victoria FACS, IM 宿主: 山羊 Polyclonal FITC
anti-Green Fluorescent Protein (GFP) antibody (GFP tag)GFP 适用: Aequorea victoria WB, ELISA, IHC, FACS, FM 宿主: 小鼠 Monoclonal 9F9-F9 GFP tag
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- 应用备注
- Anti-GFP antibody is designed to detect GFP and its variants. GFP antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.02 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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: "A small protein probe for correlated microscopy of endogenous proteins." in: Histochemistry and cell biology, Vol. 149, Issue 3, pp. 261-268, (2018) (PubMed).
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: "Mode of Surgical Injury Influences the Source of Urothelial Progenitors during Bladder Defect Repair." in: Stem cell reports, Vol. 9, Issue 6, pp. 2005-2017, (2018) (PubMed).
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: "Intracellular membranes of bacterial endospores are reservoirs for spore core membrane expansion during spore germination." in: Scientific reports, Vol. 8, Issue 1, pp. 11388, (2018) (PubMed).
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: "An RNA thermometer dictates production of a secreted bacterial toxin." in: PLoS pathogens, Vol. 16, Issue 1, pp. e1008184, (2020) (PubMed).
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- 抗原
- GFP (Green Fluorescent Protein (GFP))
- 别名
- GFP (GFP 产品)
- 物质类
- Viral Protein
- 背景
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Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP Antibody is ideal for Cell Biology, Neuroscience and Cancer research.
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
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