GFP 抗体 (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN1574093, 供应商: Log in to see
抗原
  • green fluorescent protein
  • gfp
适用
Aequorea victoria
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宿主

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克隆类型
多克隆
标记
This GFP antibody is un-conjugated
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应用范围
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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免疫原 Purified recombinant full-length GFP protein
亚型 IgG
特异性 Rabbit Anti-GFP Polyclonal Antibody reacts with either N-terminal or C-terminal GFP fusion proteins. The antibody also reacts with other variants of GFP, such as CFP, YFP, eGFP and GFPuv.
交叉反应 (详细) Rabbit Anti-GFP Polyclonal Antibody reacts with GFP fusion proteins. The antibody also reacts with other variants of GFP, such as CFP, YFP, eGFP and GFPuv.
纯化方法 Immunoaffinity chromatography
Plasmids, Primers & others Plasmids, Primers & others GFP products on genomics-online (e.g. as negative or positive controls)
抗原
别名 GFP
背景 Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. The antibodies raised against full-length GFP should also detect YFP and other variants.Rabbit Anti-GFP Polyclonal Antibody is developed in rabbit using purified recombinant full-length GFP protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
应用备注 Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunoprecipitation (IP): 2-10 µg/mg of lysateOther Applications: user-optimized
限制 仅限研究用
状态 Lyophilized
缓冲液 PBS, pH 7.4, containing 0.02 % sodium azide
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
储存条件 4 °C/-20 °C
储存方法 The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
厂商提供的图像
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN1574093) Western blot analysis of GFP fusion protein using 1 µg/mL Rabbit Anti-GFP Polyclonal ...
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN1574093) Western blot analysis of GFPuv fusion protein using 1 µg/mL Rabbit Anti-GFP Polyclona...
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN1574093) Western blot analysis of YFP fusion protein using 1 µg/mL Rabbit Anti-GFP Polyclonal ...
有引用在: Tanaka-Matakatsu, Miller, Du: "The homeodomain of Eyeless regulates cell growth and antagonizes the paired domain-dependent retinal differentiation function." in: Protein & cell, Vol. 6, Issue 1, pp. 68-78, 2015 (PubMed).

Cheng, Wang, Xu, Zhu, Hu, Huang: "Discovery of a novel small secreted protein family with conserved N-terminal IGY motif in Dikarya fungi." in: BMC genomics, Vol. 15, pp. 1151, 2015 (PubMed).

Ni, Wang, Zhang, Pang, Liu, Du: "PKD1 is downregulated in non-small cell lung cancer and mediates the feedback inhibition of mTORC1-S6K1 axis in response to phorbol ester." in: The international journal of biochemistry & cell biology, Vol. 60, pp. 34-42, 2015 (PubMed).

Land, Luo, Levin: "Functional domain analysis of the cell division inhibitor EzrA." in: PLoS ONE, Vol. 9, Issue 7, pp. e102616, 2014 (PubMed).

Chan, Seetharaman, Bagg, Selman, Zhang, Kim, Roy: "EVA-1 functions as an UNC-40 Co-receptor to enhance attraction to the MADD-4 guidance cue in Caenorhabditis elegans." in: PLoS genetics, Vol. 10, Issue 8, pp. e1004521, 2014 (PubMed).

Kwong, Kumai, Perry: "Evidence for a role of tight junctions in regulating sodium permeability in zebrafish (Danio rerio) acclimated to ion-poor water." in: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, Vol. 183, Issue 2, pp. 203-13, 2013 (PubMed).

Martínez-Vieyra, Vásquez-Limeta, González-Ramírez, Morales-Lázaro, Mondragón, Mondragón, Ortega, Winder, Cisneros: "A role for ?-dystroglycan in the organization and structure of the nucleus in myoblasts." in: Biochimica et biophysica acta, Vol. 1833, Issue 3, pp. 698-711, 2013 (PubMed).

Wang, Ketcham, Schön, Goodman, Wang, Yates, Freire, Schroer, Zheng: "Nudel/NudE and Lis1 promote dynein and dynactin interaction in the context of spindle morphogenesis." in: Molecular biology of the cell, Vol. 24, Issue 22, pp. 3522-33, 2013 (PubMed).

Kulasekara, Kamischke, Kulasekara, Christen, Wiggins, Miller: "c-di-GMP heterogeneity is generated by the chemotaxis machinery to regulate flagellar motility." in: eLife, Vol. 2, pp. e01402, 2013 (PubMed).

Li, Blissard: "Cellular VPS4 is required for efficient entry and egress of budded virions of Autographa californica multiple nucleopolyhedrovirus." in: Journal of virology, Vol. 86, Issue 1, pp. 459-72, 2011 (PubMed).

Alexander, Selman, Seetharaman, Chan, DSouza, Byrne, Roy: "MADD-2, a homolog of the Opitz syndrome protein MID1, regulates guidance to the midline through UNC-40 in Caenorhabditis elegans." in: Developmental cell, Vol. 18, Issue 6, pp. 961-72, 2010 (PubMed).

Zhang, Xing, Liu: "PUMA promotes Bax translocation by both directly interacting with Bax and by competitive binding to Bcl-X L during UV-induced apoptosis." in: Molecular biology of the cell, Vol. 20, Issue 13, pp. 3077-87, 2009 (PubMed).

Arnold, Zimmerman, Li, Lairmore, Green: "Human T-cell leukemia virus type-1 antisense-encoded gene, Hbz, promotes T-lymphocyte proliferation." in: Blood, Vol. 112, Issue 9, pp. 3788-97, 2008 (PubMed).

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