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E-cadherin 抗体

CDH1 适用: 人, 大鼠 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal CDH1-1525 unconjugated
产品编号 ABIN4949627
发货至: 中国
  • 抗原 See all E-cadherin (CDH1) 抗体
    E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
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    人, 大鼠
    宿主
    • 191
    • 69
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    小鼠
    克隆类型
    • 179
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    单克隆
    标记
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    This E-cadherin antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Protein G affinity chromatography
    免疫原
    A full length recombinant human protein was used as the immunogen for the E-Cadherin antibody.
    克隆位点
    CDH1-1525
    亚型
    IgG1 kappa
    Top Product
    Discover our top product CDH1 Primary Antibody
  • 应用备注
    Optimal dilution of the E-Cadherin antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    Store the E-Cadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
    别名
    E-Cadherin / CDH1 (CDH1 产品)
    别名
    Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, E-cadherin antibody, cdh1 antibody, si:dz180o5.2 antibody, uvo antibody, lcam antibody, E-Cad antibody, XBcad antibody, l-cam antibody, xcdh1 antibody, cdhc-A antibody, xb-cad antibody, XTCAD-1 antibody, c-cadherin antibody, cadherin-1 antibody, uvomorulin antibody, XB-cadherin antibody, arc-1 antibody, cd324 antibody, cdhe antibody, ecad antibody, AA960649 antibody, E-cad antibody, Ecad antibody, L-CAM antibody, Um antibody, cadherin 1 antibody, si:busm1-180o5.2 antibody, cadherin 1, type 1, E-cadherin (epithelial) antibody, cadherin 1, type 1 S homeolog antibody, CDH1 antibody, si:busm1-180o5.2 antibody, cdh1 antibody, Cdh1 antibody, cdh1.S antibody
    背景
    Recognizes a protein of 80-120  kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as beta-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.
    途径
    WNT signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
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