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IL1A 抗体

IL1A 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN465571
发货至: 中国
  • 抗原 See all IL1A 抗体
    IL1A (Interleukin 1 alpha (IL1A))
    适用
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    宿主
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    克隆类型
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    多克隆
    标记
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    This IL1A antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Neutralization (Neut)
    特异性
    Mouse IL-1alpha
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Murine IL-1alpha.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product IL1A Primary Antibody
  • 应用备注
    Approved: ELISA (0.5 - 2 μg/mL), Neut (0.1 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1 mg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mIL-1alpha (0.05 ng/mL), a concentration of 0 3-0.05 μg/mL of this antibody is required. ELISA: To detect mIL-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1alpha (LS-C104815) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1alpha. Western Blot: To detect mIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Mouse

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    IL1A (Interleukin 1 alpha (IL1A))
    别名
    IL1A (IL1A 产品)
    别名
    IL-1A antibody, IL1 antibody, IL1-ALPHA antibody, IL1F1 antibody, IL-6 antibody, Il-1a antibody, IL-1 alpha antibody, IL-1alpha antibody, L1A antibody, interleukin 1 alpha antibody, interleukin 6 antibody, IL1A antibody, IL6 antibody, Il1a antibody
    背景
    Name/Gene ID: IL1A
    Family: IL-1

    Synonyms: IL1A, Hematopoietin-1, IL-1 alpha, IL1, IL1-ALPHA, IL1 Alpha, Interleukin-1 alpha, IL-1A, IL1F1, Pro-interleukin-1-alpha, Preinterleukin 1 alpha, Interleukin 1, alpha
    基因ID
    3552
    途径
    NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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