IL1A 抗体
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- 抗原 See all IL1A 抗体
- IL1A (Interleukin 1 alpha (IL1A))
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IL1A antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Neutralization (Neut)
- 特异性
- Mouse IL-1alpha
- 纯化方法
- Immunoaffinity purified
- 免疫原
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E.coli derived recombinant Murine IL-1alpha.
Type of Immunogen: Cell extract - Top Product
- Discover our top product IL1A Primary Antibody
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- 应用备注
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Approved: ELISA (0.5 - 2 μg/mL), Neut (0.1 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1 mg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mIL-1alpha (0.05 ng/mL), a concentration of 0 3-0.05 μg/mL of this antibody is required. ELISA: To detect mIL-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1alpha (LS-C104815) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1alpha. Western Blot: To detect mIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - 说明
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Target Species of Antibody: Mouse
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Sterile water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- 抗原
- IL1A (Interleukin 1 alpha (IL1A))
- 别名
- IL1A (IL1A 产品)
- 别名
- IL-1A antibody, IL1 antibody, IL1-ALPHA antibody, IL1F1 antibody, IL-6 antibody, Il-1a antibody, IL-1 alpha antibody, IL-1alpha antibody, L1A antibody, interleukin 1 alpha antibody, interleukin 6 antibody, IL1A antibody, IL6 antibody, Il1a antibody
- 背景
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Name/Gene ID: IL1A
Family: IL-1
Synonyms: IL1A, Hematopoietin-1, IL-1 alpha, IL1, IL1-ALPHA, IL1 Alpha, Interleukin-1 alpha, IL-1A, IL1F1, Pro-interleukin-1-alpha, Preinterleukin 1 alpha, Interleukin 1, alpha - 基因ID
- 3552
- 途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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